Reoxygenation of hypoxic coronary smooth muscle cells amplifies growth-retarding effects of ionizing irradiation

• Published in: Circulation (New York, N.Y.) N.Y.), 2004-03, Vol.109 (8), p.1036-1040
• Main Authors: ARAB, Amina, GRUMANN, Thorsten, GUTTENBERGER, Roland, BODE, Christoph, HEHRLEIN, Christoph
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Blood and lymphatic vessels; Brachytherapy; Cardiology. Vascular system; Cell Division - radiation effects; Cell Hypoxia; Cells, Cultured - metabolism; Cells, Cultured - radiation effects; Coronary Vessels - metabolism; Coronary Vessels - radiation effects; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous; Dose-Response Relationship, Radiation; Gamma Rays; Gene Expression Regulation - radiation effects; Humans; Medical sciences; Muscle Cells - metabolism; Muscle Cells - radiation effects; Muscle, Smooth, Vascular - metabolism; Muscle, Smooth, Vascular - radiation effects; Oxygen - metabolism; Radiation Tolerance; Reactive Oxygen Species; RNA, Messenger - biosynthesis; RNA, Ribosomal, 28S - biosynthesis; RNA, Ribosomal, 28S - genetics; Vascular Endothelial Growth Factor A - biosynthesis; Vascular Endothelial Growth Factor A - genetics
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/01.CIR.0000117404.65853.AF

Hypoxic human coronary smooth muscle cells (HCSMCs) are possible targets for brachytherapy to prevent restenosis after percutaneous transluminal coronary angiography. It is unclear whether growth kinetics and gene expression of these cells undergoing gamma-irradiation are changed by reoxygenation. Hypoxic (H) and hypoxia-reoxygenated (H-R) HCSMCs were irradiated with gamma-radiation at single doses of 4, 8, and 16 Gy using a 60Co-source. Vascular endothelial growth factor gene expression of HCSMCs was dramatically suppressed in H-R versus H cells independent of the radiation dose (15+/-7% versus 2183+/-2023%, P