Molecular Marker Based Discrimination Study of Commiphora Species Distributed in India

• Published in: National Academy of Sciences, India. Proceedings. Section B. Biological Sciences India. Proceedings. Section B. Biological Sciences, 2018-12, Vol.88 (4), p.1597-1604
• Main Authors: Bishoyi, Ashok Kumar, Kavane, Aarti, Sharma, Anjali, Geetha, K. A., Samantaray, Sanghamitra
• Format: Article
• Language: English
• Subjects: Behavioral Sciences; Biomedical and Life Sciences; Commiphora; Commiphora agallocha; Commiphora caudata; Commiphora stocksiana; Commiphora wightii; Genetic relationship; Life Sciences; Nucleic Acid Chemistry; Plant Biochemistry; Primers; Random amplified polymorphic DNA; Research Article; Simple sequence repeats; Species
• ISSN: 0369-8211; 2250-1746
• DOI: 10.1007/s40011-017-0904-6

Commiphora agallocha, C. caudata, C. stocksiana and C. wightii are the four Commiphora species reported from India of which C. wightii has been extensively used in Ayurveda for its excellent medicinal values. Destructive utilization of these species needs to be checked by means of efficient conservation efforts. The present study is an attempt to species discrimination of Commiphora using analysis of molecular markers. Random Amplified Polymorphic DNAs (RAPD) and Inter Simple Sequence Repeats (ISSR) markers were used for the study considering the fact that no or very little genomic information is available in the genus. One hundred and twenty RAPD and 25 ISSR primers were screened initially from which 25 RAPD and 18 ISSR primers were used for final analysis. RAPD primers amplified 264 fragments from which 18 were monomorphic, 109 were polymorphic and 137 were unique, whereas 18 ISSR markers amplified 132 loci out of which 37 loci were polymorphic, 11 were monomorphic and 84 were unique. The cluster analysis indicated that C. agallocha was genetically distant from the other three species i.e. , C. wightii , C. stocksiana and C. caudata. Both RAPD and ISSR markers were able to generate 62 and 42 species specific markers, respectively. The specific molecular markers identified can be converted to Sequence Characterised Amplified Regions which would be helpful for molecular taxonomic study. This investigation would also be helpful to develop an insight into the genetic relationship of the limited number of Commiphora species distributed in India.