Bio-efficiency of Antifungal Lactic Acid Bacterial Isolates for Pomegranate Fruit Rot Management

Out of 360 lactic acid bacteria (LAB) from leaves, flowers, buds and fruits of pomegranate, 224 were antagonistic towards Aspergillus flavus . In all, 81.6% of antifungal LAB were cocci and 18.3% were rod shaped. Majority (39.7%) of them was obtained from flowers and 27 isolates showed strong antifu...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences, India. Section B: Biological sciences India. Section B: Biological sciences, 2018-12, Vol.88 (4), p.1477-1488
Main Authors: Gajbhiye, Milind, Kapadnis, Balu
Format: Article
Language:English
Subjects:
Antifungal activity
Behavioral Sciences
Biological control
Biomedical and Life Sciences
Cocci
Flowers
Fruit rot
Fruits
Fungicides
Inoculation
Lactic acid
Lactic acid bacteria
Life Sciences
Nucleic Acid Chemistry
Plant Biochemistry
Preservatives
Research Article
rRNA 16S
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Out of 360 lactic acid bacteria (LAB) from leaves, flowers, buds and fruits of pomegranate, 224 were antagonistic towards Aspergillus flavus . In all, 81.6% of antifungal LAB were cocci and 18.3% were rod shaped. Majority (39.7%) of them was obtained from flowers and 27 isolates showed strong antifungal activity. Antifungal isolates were identified by Gram positive card kit and 16S rRNA gene sequencing as Enterococcus faecium , E. durans , Lactococcus lactis spp. cremoris , L. lactis spp. lactis , Leuconostoc mesenteroides , Lactobacillus brevis and Pediococcus pentosaceus . All the isolates showed maximum antifungal activity at pH 6.5 and 30 °C on MRS agar. The antifungal activity of cell free supernatants from the entire isolates was same under static and shaking conditions except PFL9 that showed decreased production under shaking conditions. The isolates, PFL4, PFL9, PB6, PB119, PL14, PFR61 and PFR77 showed broad antifungal activity. PFL9 and PFR77 had the longest survival on fruits and there was no significant reduction ( P  > 0.05) in their population till 8th day post inoculation as determined under field conditions. Cell formulations of PFL9 and PFR77, and Dithane (fungicide preparation) delayed the development of rot caused by Alternaria alternata , Phomopsis varsoniana and Corynespora cassiicola with equivalent potential ( P  > 0.05). Thus, pomegranate is the source of diverse antifungal LAB which can be used as an effectual biocontrol agent in the management of fungal fruit rot of pomegranate.
ISSN:0369-8211
2250-1746
DOI:10.1007/s40011-017-0891-7