Orthodontic treatment effects on inflammatory marker profiles in saliva before and after 2 archwire changes

• Main Authors: Yamamoto Zulham, Jaafar, Ikmal Mohamad, Rohaya M A W, Abidin Intan Zarina Zainol, Senafi Sahidan, Ariffin, Zaidah Zainal, Ariffin Shahrul Hisham Zainal
• Format: Conference Proceeding
• Language: English
• Subjects: Archwires; Creatine; Intermetallic compounds; Lactate dehydrogenase; Nickel titanides; Nitric oxide; Orthodontics; Periodontal disease; Saliva; Spectrophotometry
• ISSN: 0094-243X; 1551-7616

Periodontal tissue changes exerted by external forces in orthodontic treatment allow tooth movement. The changes in periodontal tissues i.e. inflammation can be monitored using gingival crevicular fluid (GCF). GCF is a component of saliva. Saliva could be used to monitor periodontal disease progression. The use of saliva to monitor periodontal tissues changes during orthodontic treatment is still unknown. Therefore, we observed the profiles of inflammatory markers namely creatine kinase ('CK), nitric oxide (NO), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) in saliva of orthodontic patients to evaluate their importance in orthodontic treatment. A total of 21 subjects (13 female and 8 male) participated in this study. Samples were collected from gingival crevicular fluid at three period of archwire changes: baseline (M0), 2 weeks after 0.014" NiTi archwire (M1), and 2 weeks after 0.018" NiTi archwire (M2). All enzyme activities i.e. CK, LDH and AST were measured spectrophotometrically at 340 nm. Griess assay was used to measure nitric oxide level. CK activity, NO level, LDH activity and AST activity in saliva samples did not show significant differences among period of archwire changes. The use of inflammatory marker profiles in saliva may not represent the changes in periodontal tissues during orthodontic treatment.