Autologous lung cancer tissues derived gp96-peptide complex/dendritic cell vaccine induces the cytotoxic T cell response in vitro

To investigate whether autologous lung cancer tissues derived gp96-peptide complex/dendritic cell vaccine could induce peptide specific cytotoxic T lymphocyte (CTL) response in vitro. A patient's tumor-derived antigens including gp96-peptide complexes and tumor cell lysate were co cultured with...

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Bibliographic Details
Published in:Zhongguo fei ai za zhi 2002-12, Vol.5 (6), p.435
Main Authors: Shen, Chenyang, Liu, Jun, Wang, Danlei, Zhao, Hui, Zhang, Guoliang, Cai, Peng, Tong, Chunrong, Wang, Jun
Format: Article
Language:Chinese
Subjects:
Antigens
Cytotoxicity
Dendritic cells
Lung cancer
Lymphocytes
Peptides
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Summary:To investigate whether autologous lung cancer tissues derived gp96-peptide complex/dendritic cell vaccine could induce peptide specific cytotoxic T lymphocyte (CTL) response in vitro. A patient's tumor-derived antigens including gp96-peptide complexes and tumor cell lysate were co cultured with DCs derived from the same patient's bone marrow blood mononuclear cells. The various antigen/DC vaccines were used to stimulate peripheral lymphocytes. Interferon-γ (IFN-γ) level of activated lymphocytes was detected by ELISA method and the Cr51 release test was performed to evaluate the gp96-peptide specific CTL response in three kinds of target cells including the primary cultured tumor cells, PG cells and K562 cells. IFN-γ could be observed from the supernate collected in all antigen groups after the cognate T lymphocytes were stimulated by various vaccines. The concentration of IFN-γ induced by gp96-peptide complexes/DC vaccine was higher than that of other groups. In addition, the killing effect of the activated T
ISSN:1009-3419
1999-6187
DOI:10.3779/cjlc.v5i6.3031