Hepatoprotective activity of Uncaria tomentosa extract against sub-chronic exposure to fipronil in male rats

• Published in: Environmental science and pollution research international 2019, Vol.26 (1), p.199-207
• Main Authors: Elgawish, Rania Abdelrahman, Abdelrazek, Heba M. A., Ismail, Shimaa A. A., Loutfy, Naglaa M., Soliman, Mohamed T. A.
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - metabolism; Antioxidants - pharmacology; Antioxidants - therapeutic use; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Body weight gain; Cat's Claw - chemistry; Cell Line; Chemical and Drug Induced Liver Injury - metabolism; Chemical and Drug Induced Liver Injury - prevention & control; Chronic exposure; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental Pollutants - adverse effects; Environmental science; Enzymes; Fipronil; Histopathology; Immunohistochemistry; Insecticides; Insecticides - adverse effects; Interleukin 6; Interleukin-6 - metabolism; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Liver; Liver - drug effects; Liver - metabolism; Liver - pathology; Male; Malondialdehyde - metabolism; NF-kappa B - metabolism; NF-κB protein; Oxidative stress; Oxidative Stress - drug effects; Peroxidation; Pesticides; Phytotherapy; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Protective Agents - pharmacology; Protective Agents - therapeutic use; Pyrazoles - adverse effects; Rats; Rats, Wistar; Research Article; Rodents; Tumor Necrosis Factor-alpha - antagonists & inhibitors; Tumor necrosis factor-α; Uncaria tomentosa; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-018-3615-5

The effects of fipronil (FPN) on the liver of rats were studied. Rats ( n  = 6) were treated with 9.7 mg/kg (1/10 of FPN LD 50 ), and other rats ( n  = 6) received 120 mg/kg of 10% Uncaria tomentosa extract, while a mixture of 9.7 mg/kg FPN and 120 mg/kg of 10% Uncaria tomentosa extract were administered orally to the rats ( n  = 6) daily for 6 weeks. Body, hepatic weights, liver enzymes, and lipid profile were determined. Hepatic activities of MDA, TNO, TAC, TNF-α, and IL-6 in liver homogenate were measured. Immunohistochemistry of NF-kB and liver histopathology were performed. Fipronil-treated rats had a significant ( P  = 0.02) lower weight gain. Moreover, relative liver weight was significantly ( P  = 0.003) increased in FPN-treated rats. Rats administrated with FPN exhibited a significantly ( P  = 0.02) higher liver enzymes and promoted levels of MDA, TNO, TNF-α, and IL-6 ( P