Development of drug resistance in the population of colon cancer cells under the effect of multifunctional protein YB-1

• Published in: Bulletin of experimental biology and medicine 2007-04, Vol.143 (4), p.463-466
• Main Authors: Vaiman, A V, Stromskaya, T P, Rybalkina, E Yu, Sorokin, A V, Ovchinnikov, L P, Stavrovskaya, A A
• Format: Article
• Language: English
• Subjects: ATP Binding Cassette Transporter, Subfamily B, Member 1 - genetics; ATP Binding Cassette Transporter, Subfamily B, Member 1 - metabolism; Cell Line, Tumor; Cell Nucleus - metabolism; Colonic Neoplasms - genetics; Colonic Neoplasms - metabolism; Colonic Neoplasms - pathology; Drug Resistance, Multiple - drug effects; Drug Resistance, Multiple - genetics; Drug Resistance, Neoplasm - genetics; Gene Expression Regulation, Neoplastic - drug effects; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Humans; Microscopy, Fluorescence; Protein Transport - drug effects; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Vinblastine - pharmacology; Y-Box-Binding Protein 1 - genetics; Y-Box-Binding Protein 1 - metabolism; Y-Box-Binding Protein 1 - physiology
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-007-0157-0

The effects of YB-1 gene on the expression level of P-glycoprotein and drug resistance of tumor cells were studied in cultured HCT116 colon cancer cells. Transitory transfection of chimeric YB-1/GFP gene rendered HCT116 cells a selective advantage in a medium with vinblastine, which caused translocation of the chimeric protein into cell nuclei. This was paralleled by an increase in the expression of P-glycoprotein (multiple drug resistance protein).