BIOASSAY-GUIDED ISOLATION OF OCHROLIFUANINE: A BISINDOLE ALKALOID OF DYERA COSTULATA ACTIVE ON TRYPANOSOMA BRUCEI BRUCEI

Dyera costulata, locally known as jelutong, have shown promising in vitro inhibitory activity against Trypanosoma brucei brucei strain BS221. The methanolic leaf extract, evaluated using Alamar Blue Assay, showed strong antitrypanosomal activity (IC50 0.58 ± 0.01 μg mL−1) and high selectivity index...

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Bibliographic Details
Published in:Journal of tropical forest science 2018-01, Vol.30 (4), p.560-569
Main Authors: Norhayati, I, Muhd-Haffiz, J, Getha, K, Mohd-Ilham, A, Nurhayati, ZA, Lili-Sahira, H, Muhd-Syamil, A, Mohd-Hafidz-Hadi, A
Format: Article
Language:English
Subjects:
Alkaloids
Antimalarial agents
Antimicrobial activity
Bioassays
Chromatography
Diabetes
Drugs
Dyera costulata
Flowers & plants
Fractionation
Herbal medicine
Leaves
Metabolites
Natural products
NMR
Nuclear magnetic resonance
Parasitic diseases
Pharmaceutical industry
Pharmaceutical sciences
Protozoa
Selectivity
Spectral analysis
Two dimensional analysis
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Summary:Dyera costulata, locally known as jelutong, have shown promising in vitro inhibitory activity against Trypanosoma brucei brucei strain BS221. The methanolic leaf extract, evaluated using Alamar Blue Assay, showed strong antitrypanosomal activity (IC50 0.58 ± 0.01 μg mL−1) and high selectivity index (SI value 169.5) towards the protozoa. While, the hexane leaf extract was moderately active (IC50 4.4 ± 0.05 μg mL−1; SI value of 22.7). Total alkaloids extracted from the active methanolic fraction showed strong activity and high selectivity (IC50 0.08 ± 0.07 μg mL−1, SI value 890) towards T. b. brucei. Bioassay-guided fractionation of the total alkaloid extract yielded an active compound 1 (IC50 0.06 ± 0.03 μg mL−1, SI value 76.3). Compound 1 was identified as ochrolifuanine from the bisindole alkaloid group, based on spectral analysis (MS, 1D and 2D NMR). The presence of alkaloids in D. costulata leaves may be indicative of the various bioactivities observed in this plant. Previously, ochrolifuanine has been reported for antimalarial and antimicrobial activity. This study reports, for the first time, on the antitrypanosomal activity of ochrolifuanine from D. costulata.
ISSN:0128-1283
2521-9847
DOI:10.26525/jtfs2018.30.4.560569