Inhibitory Effect of [alpha]-Tocopherol on Benzo(a)pyrene-Induced CYPA1 Activity in Rat Liver

• Published in: Bulletin of experimental biology and medicine 2005-11, Vol.140 (5), p.517
• Main Authors: Sidorova, Yu A, A. Yu. Grishanova
• Format: Article
• Language: English
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-006-0012-8

Issue Title: This issue is a translation of Byulleten' Eksperimental'noi Biologii i Meditsiny (Bulletin of Experimental Biology and Medicine) and Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine) Combined treatment with benzo(a)pyrene (classic inductor of cytochromes P450 of subfamily 1A, CYP1A1 and CYP1A2) and α-tocopherol decreased benzo(a)pyrene-induced CYP1A1 activity in rat liver. Activities of CYP1A2, NADPH-cytochrome P450 reductase, and glutathione S-transferase remained unchanged under these conditions. Addition of α-tocopherol to benzo(a)pyrene-induced microsomes in vitro decreased activity of CYP1A1. Immunoblotting of proteins in liver microsomes with antibodies against CYP1A1 did not reveal differences in CYP1A1 protein content in the liver of rats receiving benzo(a)pyrene alone or in combination with α-tocopherol. The in vivo decrease in benzo(a)pyrene-induced CYP1A1 activity did not result from free radical-produced damage to CYP1A1. The inhibition of benzo(a)pyrene-induced CYP1A1 activity with α-tocopherol is probably realized at the posttranslational level.