Cathepsin X-mediated [beta]2 integrin activation results in nanotube outgrowth

Membrane nanotubes were recently described as a new principle of cell-cell communication enabling complex and specific messaging to distant cells. Calcium fluxes, vesicles, and cell-surface components can all traffic between cells connected by nanotubes. Here we report for the first time the mechani...

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Published in:Cellular and molecular life sciences : CMLS 2009-03, Vol.66 (6), p.1126
Main Authors: Obermajer, N, Jevnikar, Z, Doljak, B, Sadaghiani, A M, Bogyo, M, Kos, J
Format: Article
Language:English
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Cellular biology
Enzymes
Immune response
Lymphocytes
Molecular biology
Nanotechnology
Nanotubes
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container_title Cellular and molecular life sciences : CMLS
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creator Obermajer, N
Jevnikar, Z
Doljak, B
Sadaghiani, A M
Bogyo, M
Kos, J
description Membrane nanotubes were recently described as a new principle of cell-cell communication enabling complex and specific messaging to distant cells. Calcium fluxes, vesicles, and cell-surface components can all traffic between cells connected by nanotubes. Here we report for the first time the mechanism of membrane nanotube formation in T cells through LFA-1 (CD11a/CD18; αLβ2) integrin activation by the cysteine protease cathepsin X. Cathepsin X is shown to induce persistent LFA-1 activation. Cathepsin X-upregulated T cells exhibit increased homotypic aggregation and polarized, migration-associated morphology in 2D and 3D models, respectively. In these cells, extended uropods are frequently formed, which subsequently elongate to nanotubes connecting T lymphocytes. Our results demonstrate that LFA-1 activation with subsequent cytoskeletal reorganization induces signal transmission through a physically connected network of T lymphocytes for better coordination of their action at various stages of the immune response. [PUBLICATION ABSTRACT]
doi_str_mv 10.1007/s00018-009-8829-8
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source Open Access: PubMed Central; Springer Link
subjects Cellular biology
Enzymes
Immune response
Lymphocytes
Molecular biology
Nanotechnology
Nanotubes
title Cathepsin X-mediated [beta]2 integrin activation results in nanotube outgrowth
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