Ascorbate modulation of H2O2 and camptothecin-induced cell death in Jurkat cells

The effect of ascorbate on cell death was examined in Jurkat cells (human T-cell leukemia) by incubation with dehydroascorbate (DHA), which is rapidly taken up by cells and efficiently reduced to ascorbate. Apoptosis was evaluated by caspase-3 activity in cell extracts and flow cytometry of annexin...

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Bibliographic Details
Published in:Cancer chemotherapy and pharmacology 2004-10, Vol.54 (4), p.315-321
Main Authors: SANE, Alain-Théophile, CANTIN, André M, PAQUETTE, Benoit, WAGNER, J. Richard
Format: Article
Language:English
Subjects:
Antineoplastic agents
Biological and medical sciences
Cell death
Medical sciences
Pharmacology. Drug treatments
Tumors
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Summary:The effect of ascorbate on cell death was examined in Jurkat cells (human T-cell leukemia) by incubation with dehydroascorbate (DHA), which is rapidly taken up by cells and efficiently reduced to ascorbate. Apoptosis was evaluated by caspase-3 activity in cell extracts and flow cytometry of annexin V-labeled cells. In parallel, necrosis was estimated by the release of lactate dehydrogenase. Minor effects on cell death were observed when Jurkat cells were incubated with either DHA alone (100-1,000 [mu]M) or a single dose of 10 [mu]M H
ISSN:0344-5704
1432-0843
DOI:10.1007/s00280-004-0828-8