Biotransformation of ginsenosides F4 and Rg6 in zebrafish

Ginsenosides F 4 and Rg 6 (GF 4 and GRg 6 ), two main active components of steamed notoginseng or red ginseng, are dehydrated disaccharide saponins. In this work, biotransformation of ginsenosides F 4 and Rg 6 in zebrafish was investigated by qualitatively identifying their metabolites and then prop...

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Bibliographic Details
Published in:Journal of asian natural products research 2018-07, Vol.20 (7), p.686-696
Main Authors: Shen, Wen-Wen, Zhang, Hai-Xia, Qiu, Shou-Bei, Wei, Ying-Jie, Zhu, Fen-Xia, Wang, Jing, Wang, Dan-Dan, Jia, Xiao-Bin, Tang, Dao-Quan, Chen, Bin
Format: Article
Language:English
Subjects:
and Rg
Biotransformation
Computer simulation
Danio rerio
Dehydration
Disaccharides
Ginseng
Ginsenosides
Ginsenosides F
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metabolic pathways
Metabolism
Metabolites
Quadrupoles
Rhamnose
Saponins
Sulfation
UPLC-Q-TOF/MS
Zebrafish
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Summary:Ginsenosides F 4 and Rg 6 (GF 4 and GRg 6 ), two main active components of steamed notoginseng or red ginseng, are dehydrated disaccharide saponins. In this work, biotransformation of ginsenosides F 4 and Rg 6 in zebrafish was investigated by qualitatively identifying their metabolites and then proposing their possible metabolic pathways. The prediction of possible metabolism of ginsenosides F 4 and Rg 6 using zebrafish model which can effectively simulate existing mammals model was early and quickly performed. Metabolites of ginsenosides F 4 and Rg 6 after exposing to zebrafish for 24 h were identified by Ultraperformance Liquid Chromatography/Quadrupole-Time-of-Flight Mass Spectrometry. A total of 8 and 6 metabolites of ginsenosides F 4 and Rg 6 were identified in zebrafish, respectively. Of these, 7 and 5, including M1, M3−M5, M7−M9 and N1 (N5), N2, N4 (N9), N7−N8 were reported for the first time as far as we know. The mechanisms of their biotransformation involved were further deduced to be desugarization, glucuronidation, sulfation, dehydroxylation, loss of C-17 and/or C-23 residue pathways. It was concluded that loss of rhamnose at position C-6 and glucuronidation at position C-3 in zebrafish were considered as the main physiologic and metabolic processes of ginsenosides F 4 and ginsenosides Rg 6 , respectively.
ISSN:1028-6020
1477-2213
DOI:10.1080/10286020.2017.1307184