Additive apoptotic effect of STI571 with the cytoprotective agent amifostine in K-562 cell line

To study the apoptotic effect of the 2-phenylaminopyrimidine derivative STI571 in combination with antioxidant agents on K-562 cell line derived from a Philadelphia chromosome-positive chronic myeloid leukemia patient. K-562 (BCR/ABL+), U-937, and HL60 (BCR/ABL-) leukemic cell lines were incubated w...

Full description

Saved in:
Bibliographic Details
Published in:Cancer chemotherapy and pharmacology 2005-06, Vol.55 (6), p.602-608
Main Authors: VELLON, Luciano, GONZALEZ-CID, Marcela, DE CAMPOS NEBEL, Marcelo, LARRIPA, Irene
Format: Article
Language:English
Subjects:
Amifostine - pharmacology
Antineoplastic agents
Antineoplastic Agents - pharmacology
Antioxidants - pharmacology
Apoptosis - drug effects
Benzamides
Biological and medical sciences
Cell Cycle - drug effects
Cell Proliferation - drug effects
Comet Assay
Cytoprotection
Drug Screening Assays, Antitumor
Drug Synergism
HL-60 Cells
Humans
Imatinib Mesylate
K562 Cells
Medical sciences
Membrane Potentials - drug effects
Mitochondria - drug effects
Mitochondria - physiology
Pharmacology. Drug treatments
Piperazines
Pyrimidines - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To study the apoptotic effect of the 2-phenylaminopyrimidine derivative STI571 in combination with antioxidant agents on K-562 cell line derived from a Philadelphia chromosome-positive chronic myeloid leukemia patient. K-562 (BCR/ABL+), U-937, and HL60 (BCR/ABL-) leukemic cell lines were incubated with STI571 and the antioxidant agents catalase, glutathione, superoxide dismutase, and amifostine (AMI). Apoptotic effect was analyzed by morphological and flow cytometric criteria. STI571 at concentrations higher than 0.25 mumol L(-1) produced apoptosis (P
ISSN:0344-5704
1432-0843
DOI:10.1007/s00280-004-0952-5