Characterization and activity of endophytic bacteria from 'Prata Anã' banana crop (Musa sp., AAB) 1

The objective of this study was to characterize banana tree endophytic bacteria at genus and species level and to determine the metabolic reactions associated with the nitrogen transformations. The identification at genus and species levels was performed using the partial sequencing of the rDNA 16S...

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Bibliographic Details
Published in:Revista Ceres 2018-09, Vol.65 (5), p.381-387
Main Authors: Pereira, Débora Francine Gomes Silva, Nietsche, Silvia, Xavier, Adelica Aparecida, de Souza, Suzane Ariádina, Costa, Márcia Regina, Duarte, Anunciene Barbosa
Format: Article
Language:English
Subjects:
Agricultural biotechnology
Bacillus
Bacteria
Biochemical tests
Deoxyribonucleic acid
DNA
Endophytes
Genetic engineering
Identification
Klebsiella
Metabolism
Microorganisms
Molecular biology
Musaceae
NifH gene
Nitrate reductase
Nitrate reduction
Nitrates
Nitrogen
Nitrogen fixation
Nitrogenase
Nitrogenation
Plant growth
Reductases
Reduction
rRNA 16S
Species
Studies
Trees
Urease
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Summary:The objective of this study was to characterize banana tree endophytic bacteria at genus and species level and to determine the metabolic reactions associated with the nitrogen transformations. The identification at genus and species levels was performed using the partial sequencing of the rDNA 16S region. The assimbyotic nitrogen fixation, the reduction of nitrate and the production of urease were in vitro evaluated. The DNA of the bacterial isolates was also amplified to verify the presence of the nifH, nirK and nirS regions. Biochemical tests were performed in a complete randomized design; the treatments consisted of 39 bacterial isolates with three replications. Sequence analysis enabled the identification of four genera: Bacillus, Rhizobium, Klebsiella and Enterobacter. The Bacillus genus occurred more frequently, nine species were identified. By evaluating the results of biochemical tests, it was observed that three isolates showed multiple abilities: growth in NFb medium, nitrate reduction and production of urease. The isolates belong to the genus Bacillus and of the species subtilis, thuringienses and amyloliquefaciens. Approximately 12.5% of the isolates amplified the region corresponding to the nifH gene, 7.5% amplified gene nirK and 3.9% amplified the nirS gene. Endophytic bacteria evaluated in the present study showed in vitro activity for urease, nitrate reductase enzymes, however, relevant nitrogenase activity was not observed.
ISSN:0034-737X
2177-3491
DOI:10.1590/0034-737X201865050001