Quantum dots tethered membrane type 3 matrix metalloproteinase-targeting peptide for tumor optical imaging

Membrane type matrix metalloproteinases (MT-MMPs) play important roles in malignant tumor progression through the degradation of the extracellular matrix and signal transduction. However, a member of the family, MT3-MMP, has attracted the least concern compared with other MT-MMPs. Here, a novel MT3-...

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Bibliographic Details
Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2018-12, Vol.6 (46), p.7719-7727
Main Authors: Ren, Li, Li, Qinglan, Ma, Zheng, Wang, Ye, Li, Haolong, Shen, Liqiao, Yu, Jihong, Fang, Xuexun
Format: Article
Language:English
Subjects:
Biophysics
Calorimetry
Cancer
Data processing
Enzyme-linked immunosorbent assay
Extracellular matrix
Flow cytometry
Matrix metalloproteinase
Matrix metalloproteinases
Medical imaging
Metalloproteinase
Peptides
Phages
Protein purification
Protein structure
Proteins
Quantum dots
Signal transduction
Spectroscopy
Titration
Titration calorimetry
Transduction
Tumor cells
Tumors
Zeta potential
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Summary:Membrane type matrix metalloproteinases (MT-MMPs) play important roles in malignant tumor progression through the degradation of the extracellular matrix and signal transduction. However, a member of the family, MT3-MMP, has attracted the least concern compared with other MT-MMPs. Here, a novel MT3-MMP-targeting peptide with high affinity and specificity has been developed by a phage-display peptide screening technology and multiple biophysics measurements, including single-molecule recognition force spectroscopy and isothermal titration calorimetry. The binding peptides are conjugated on the surface of CdSe/ZnS quantum dots (QDs) and consequently acted as a ligand that specifically targets MT3-MMP overexpressed tumor cells. The imaging nanoprobes used QDs as the photographic developer for optical imaging in vivo . The nanoprobes exhibited a desirable targeting effect and generated good biodistribution profiles for visualization and imaging of MT3-MMP overexpressed tumor. The peptide could be useful to evaluate the distribution and expression of MT3-MMP. Furthermore, the peptide-functionalized QDs show potential application for cancer diagnosis. An MT3-MMP-targeting nanoprobe shows potential application for cancer diagnosis.
ISSN:2050-750X
2050-7518
DOI:10.1039/c8tb02025f