Screening of Sesame Cultivars with Scant Albumins by an Immunological Approach

In this study a detection method for 2S albumin (14-kDa albumin) among the sesame allergens was developed. Polyclonal antibodies against 14-kDa albumin from sesame seeds (anti 14-kDa albumin antibody) were prepared. The anti 14-kDa albumin antibody absorbed with acetone powder from soybean seeds spe...

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Bibliographic Details
Published in:Food biotechnology 2018-10, Vol.32 (4), p.273-285
Main Authors: Kishikawa, Akihiro, Inoue, Maya, Tarutani, Shotaro, Okunishi, Takumi, Ina, Rie, Yoshida, Kazushi, Takada, Naoyuki, Yoshida, Motonobu
Format: Article
Language:English
Subjects:
Acetone
Albumin
Albumins
Allergens
Antibodies
Breeding
Corn
Cultivars
Enzyme-linked immunosorbent assay
immunological approach
Immunology
Polyclonal antibodies
Powder
Screening
Seeds
sesame allergy
Sesamum indicum
Soybeans
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Summary:In this study a detection method for 2S albumin (14-kDa albumin) among the sesame allergens was developed. Polyclonal antibodies against 14-kDa albumin from sesame seeds (anti 14-kDa albumin antibody) were prepared. The anti 14-kDa albumin antibody absorbed with acetone powder from soybean seeds specifically reacted with sesame albumin, and did not react with albumins from soybean, corn, Arabidopsis. The absorbed anti 14-kDa albumin antibody was used as a probe for screening sesame varieties with scant albumins by indirect enzyme-immunosorbent assay (ELISA). We isolated accession number (Acc. No.) 526 with one-third of albumin amounts compared to standard variety Acc. No. 800. This method will be useful in breeding sesame cultivars with low sesame allergens
ISSN:0890-5436
1532-4249
DOI:10.1080/08905436.2018.1519445