Selection and evaluation of the binding of aptamers against NS5 Zika virus using fluorescence spectroscopy

The Zika virus (ZIKV) is an Arbovirus belonging to the family Flaviviridae, genus Flavivirus, whose transmission occurs mainly by bites of mosquitoes of the genus Aedes. Although clinical manifestations of the disease are usually mild, increasing evidence is associated with ZIKV infection with incre...

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Bibliographic Details
Main Authors: Alves, L. N., Abalo, A. A., Argondizzo, A. P. C., Rocha, H. F., Silva, D., Cortez, C. M., Missailidis, S.
Format: Conference Proceeding
Language:English
Subjects:
Abnormalities
Antibodies
Diagnosis
Fluorescence
Infections
Mosquitoes
Proteins
Viruses
Zika virus
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Description
Summary:The Zika virus (ZIKV) is an Arbovirus belonging to the family Flaviviridae, genus Flavivirus, whose transmission occurs mainly by bites of mosquitoes of the genus Aedes. Although clinical manifestations of the disease are usually mild, increasing evidence is associated with ZIKV infection with increased congenital neurological abnormalities and neuropathic disorders. The laboratory diagnosis of the infection is based on molecular tests and viral isolation since serological tests usually present cross-reaction with other Flaviviruses. A promising solution for rapid and early diagnosis of infection is the development of nucleic acid aptamers capable of specifically interacting, recognizing infectious agents or detecting antibodies. Following this premise, this study aims to identify specific aptamers for NS5 protein of ZIKV, in order to use them in serological tests.
ISSN:0094-243X
1551-7616
DOI:10.1063/1.5079160