Protective effect of a novel rice extract against ethanol-induced gastric mucosal injury in rat

• Published in: Digestive diseases and sciences 2007-02, Vol.52 (2), p.434-441
• Main Authors: MATSUHASHI, Tamotsu, OTAKA, Michiro, WATANABE, Sumio, ODASHIMA, Masaru, JIN, Mario, KOMATSU, Koga, WADA, Isao, HORIKAWA, Youhei, OHBA, Reina, OYAKE, Jinko, HATAKEYAMA, Natsumi
• Format: Article
• Language: English
• Subjects: Animals; Anti-Ulcer Agents - pharmacology; Biological and medical sciences; Cell Line; Cell Survival - drug effects; Cytoprotection - drug effects; Dinoprostone - metabolism; Disease Models, Animal; Dose-Response Relationship, Drug; Epithelial Cells - drug effects; Ethanol - toxicity; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Gastric Acid - secretion; Gastric Mucosa - drug effects; Gastric Mucosa - metabolism; Gastric Mucosa - pathology; HSP72 Heat-Shock Proteins - metabolism; Male; Oryza; Plant Extracts - pharmacology; Rats; Rats, Sprague-Dawley; Stomach Ulcer - chemically induced; Stomach Ulcer - metabolism; Stomach Ulcer - pathology; Stomach Ulcer - physiopathology; Stomach Ulcer - prevention & control; Time Factors; TRPV Cation Channels - metabolism; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Wound Healing - drug effects
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-006-9571-9

The aim of this study was to investigate the protective action of rice extract on ethanol-induced mucosal damage in vivo and wound healing of epithelial cells in vitro. Also, the effect of rice extract on gastric mucosal prostaglandin E(2) level, HSP72 expression, gastric acid secretion, and contribution of vanilloid receptor-mediated action was studied. In addition, using cultured gastric mucosal cells (RGM-1), the effect of rice extract on cytoprotection and wound healing of epithelial cells was evaluated. Rice extract significantly reduced gastric mucosal damage produced by ethanol in vivo, and heat treatment (80 degrees C, 3 min) of this agent did not alter its protective effect. Rice extract also protected RGM-1 from ethanol-induced damage in a dose-dependent manner. Rice extract accelerated wound healing of gastric epithelial cells. Our results demonstrate that rice extract could be an alternative ulcer treatment that provides cytoprotection and enhancement of wound healing not dependent on acid secretion, prostaglandin E(2) level, HSP72 expression, or vanilloid receptors.