Influence of disease site and activity on peripheral neutrophil function in inflammatory bowel disease

Reactive oxygen species, released by phagocytes, are involved in tissue injury in inflammatory bowel diseases. The aim of our study was to evaluate peripheral neutrophil function in patients with ulcerative colitis (N = 66) and Crohn's disease (N = 62) with respect to disease activity and exten...

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Bibliographic Details
Published in:Digestive diseases and sciences 2000-08, Vol.45 (8), p.1594-1600
Main Authors: D'ODORICO, Anna, D'INCA, Renata, MESTRINER, Cinzia, DI LEO, Vincenza, FERRONATO, Antonio, STURNIOLO, Giacomo Carlo
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Colitis, Ulcerative - physiopathology
Crohn Disease - physiopathology
Digestive system
Female
Humans
Ileal Diseases - physiopathology
Inflammatory bowel disease
Investigative techniques, diagnostic techniques (general aspects)
Luminescent Measurements
Male
Medical sciences
N-Formylmethionine Leucyl-Phenylalanine - pharmacology
Neutrophil Activation
Neutrophils - physiology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Reactive Oxygen Species
Tetradecanoylphorbol Acetate - pharmacology
Zymosan - pharmacology
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Summary:Reactive oxygen species, released by phagocytes, are involved in tissue injury in inflammatory bowel diseases. The aim of our study was to evaluate peripheral neutrophil function in patients with ulcerative colitis (N = 66) and Crohn's disease (N = 62) with respect to disease activity and extent, using chemiluminometry after three stimuli. Twenty-seven healthy subjects were enrolled as controls. Neutrophils from ulcerative colitis and Crohn's disease patients had a significantly higher response than those from controls following phorbol myristate acetate (86.6 +/- 6.5, 173.8 +/- 11.9, 167.5 +/- 12.2 mV, P < 0.0001), formyl-methionyl-leucyl-phenylalanine (39.5 +/- 3.4, 41.3 +/- 2.7, 58.6 +/- 4.7 mV, P < 0.001), and zymosan (142.6 +/- 10.4, 223.7 +/- 8.9, 231.2 +/- 9.5 mV, P < 0.0001) administration. The increased response was observed during both active disease and remission. The highest chemiluminescence values were found in patients with active ulcerative pancolitis and ileal Crohn's disease. The activation of circulating neutrophils may indicate persistent intestinal inflammation or may be triggered by luminal factors even in the absence of symptoms.
ISSN:0163-2116
1573-2568
DOI:10.1023/A:1005521212948