Application of Tissue Microarrays to Study the Influence of Dexamethasone on NF-[kappa]B Expression of Pancreas in Rat with Severe Acute Pancreatitis

• Published in: Digestive diseases and sciences 2008-02, Vol.53 (2), p.571
• Main Authors: Zhang, Xi Ping, Zhang, Ling, Xu, Hong Miao, Xu, Yan Ping, Cheng, Qi Hui, Wang, Jian Mei, Shen, Hai Ping
• Format: Article
• Language: English
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-007-9867-4

To discuss the influence of dexamethasone on NF-κB expression of pancreas in rat with severe acute pancreatitis (SAP). Ninety rat SAP models were divided into the model group and dexamethasone treatment group with 45 rats in each group; another healthy 45 rats were selected to be the sham operation group. The groups were divided into the 3, 6 and 12 h group with 15 rats in each group. The survivals, pancreas pathological changes were observed 3, 6 and 12 h after operation. The changes in expression levels of NF-κB protein of pancreas tissue microarray were observed. The treatment group was significantly lower than the model group at 3 and 6 h (P < 0.05) and than the model group at 12 h in pancreas pathological scores (P < 0.01). The expression level of NF-κB protein of pancreas head of the treatment group was significantly less than that of the model group at 3 h (P < 0.01). The alleviation of pancreatic tissue injury by dexamethasone during SAP might be closely related to its role in inhibiting NF-κB expression and regulating cytokines. The advantages of tissue microarrays in pancreatitis pathological examination include time and energy savings, high efficiency and representative results. [PUBLICATION ABSTRACT]