Expression of protooncogene c-kit and its ligand stem cell factor (SCF) in gastric carcinoma cell lines

We examined 13 human gastric carcinoma cell lines for the expression of both c-kit and stem cell factor (SCF). Expression of mRNAs was detected by both Northern blot analysis and reverse transcriptase-polymerase chain reaction (RT-PCR), and expression of translated proteins was detected by western b...

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Bibliographic Details
Published in:Digestive diseases and sciences 1998, Vol.43 (1), p.8-14
Main Authors: HASSAN, S, KINOSHITA, Y, CHIBA, T, KAWANAMI, C, KISHI, K, MATSUSHIMA, Y, OHASHI, A, FUNASAKA, Y, OKADA, A, MAEKAWA, T, HE-YAO, W
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Northern
Blotting, Western
Cell Line
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Ligands
Medical sciences
Proto-Oncogene Proteins c-kit - analysis
RNA, Messenger - analysis
Stem Cell Factor - analysis
Stomach Neoplasms - genetics
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
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Summary:We examined 13 human gastric carcinoma cell lines for the expression of both c-kit and stem cell factor (SCF). Expression of mRNAs was detected by both Northern blot analysis and reverse transcriptase-polymerase chain reaction (RT-PCR), and expression of translated proteins was detected by western blotting. Using RT-PCR we confirmed the expression of c-kit in five (ECC12, TMK1, MKN7, GCIY, and HGC27) cell lines. Northern blot analysis showed coexpression of both c-kit and SCF in ECC12 and expression of SCF in five other (MKN74, MKN1 OKAJIMA, KATOIII, and TMK1) cell lines. SCF stimulated both tyrosine phosphorylation of c-kit and growth of ECC12, whereas it did not stimulate those of GCIY. The sizes of c-kit transcript and protein in GCIY were slightly smaller than those of the reported ones, suggesting the presence of a biologically inactive truncated form of c-kit in GCIY. The present study suggests that c-kit/SCF system might play an important role in the carcinogenesis and tumor growth of ECC12 and that the truncated form of c-kit in GCIY might not be associated with malignant transformation.
ISSN:0163-2116
1573-2568
DOI:10.1023/A:1018851415704