Protease activity in a hapten-induced model of ulcerative colitis in rats

Inflammatory bowel disease (IBD) is a painful and debilitating condition affecting the mucosal lining of the colon and other areas of the gastrointestinal tract. IBD generally falls into two major categories: ulcerative colitis (UC) and Crohn's disease. We have utilized dinitrobenzenesulfonic a...

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Bibliographic Details
Published in:Digestive diseases and sciences 1997-09, Vol.42 (9), p.1969-1980
Main Authors: HAWKINS, J. V, EMMEL, E. L, FEUER, J. J, NEDELMAN, M. A, HARVEY, C. J, KLEIN, H. J, ROZMIAREK, H, KENNEDY, A. R, LICHTENSTEIN, G. R, BILLINGS, P. C
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Colitis, Ulcerative - chemically induced
Colitis, Ulcerative - enzymology
Colon - drug effects
Colon - enzymology
Colon - pathology
Dinitrofluorobenzene - analogs & derivatives
Disease Models, Animal
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Intestinal Mucosa - drug effects
Intestinal Mucosa - enzymology
Intestinal Mucosa - pathology
Medical sciences
Other diseases. Semiology
Rats
Rats, Sprague-Dawley
Serine Endopeptidases - metabolism
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
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Summary:Inflammatory bowel disease (IBD) is a painful and debilitating condition affecting the mucosal lining of the colon and other areas of the gastrointestinal tract. IBD generally falls into two major categories: ulcerative colitis (UC) and Crohn's disease. We have utilized dinitrobenzenesulfonic acid (DNBS) to induce experimental UC in rats. Histopathologic analysis indicates that DNBS induces a condition in animals similar to human UC. Biochemical results revealed 6- to 10-fold elevated levels of serine protease activity in colon tissue from animals with UC as compared with matched controls. We also observed elevated levels of protease activity in tissue samples obtained from human patients with UC. Hence, our results demonstrate that protease activity is increased in rodent and human UC. These proteases may play a significant role in destruction of colonic tissue in IBD. Protease inhibitors that target serine proteases may be useful pharmacological agents to limit tissue destruction in IBD.
ISSN:0163-2116
1573-2568
DOI:10.1023/A:1018887832465