An ultrathin iron-porphyrin based nanocapsule with high peroxidase-like activity for highly sensitive glucose detection

• Published in: Nanoscale 2018-12, Vol.1 (47), p.22155-2216
• Main Authors: Fan, Xiaotong, Tian, Ruizhen, Wang, Tingting, Liu, Shengda, Wang, Liang, Xu, Jiayun, Liu, Junqiu, Ma, Min, Wu, Zhengzhi
• Format: Article
• Language: English
• Subjects: Biosensing Techniques; Catalysis; Colorimetry; Glucose; Glucose - analysis; Glucose Oxidase - chemistry; Humans; Hydrogen Peroxide - chemistry; Iron; Iron - chemistry; Limit of Detection; Microscopy, Electron, Transmission; Nanocapsules - chemistry; Oxidation-Reduction; Oxygen - chemistry; Peroxidase; Peroxidase - chemistry; Peroxidases - chemistry; Polymers - chemistry; Porphyrins - chemistry
• ISSN: 2040-3364; 2040-3372
• DOI: 10.1039/c8nr07288d

For the first time, an ultrathin iron-porphyrin based polymer nanocapsule with multiple peroxidase-like catalytic centers was constructed by covalently assembling iron-porphyrin monomers; this nanocapsule with a single molecule thickness shell acted as a highly efficient artificial enzyme for mimicking peroxidase. On the basis of the peroxidase-like activity of Fe-TPyP based nanocapsules (Fe-TPyP NCs), a highly sensitive colorimetric sensor for glucose determination was fabricated, the limit of detection was found to be as low as 0.098 μM. This study provided a novel strategy for developing artificial enzymes based on covalently assembled nanostructures. Furthermore, the colorimetric sensor for glucose determination showed potential applications in biomedicine and biology. A peroxidase mimic was firstly fabricated using a covalent assembly strategy, which can be used for highly sensitive glucose detection.