Effects of minocycline and rapamycin in gamma-irradiated human embryonic stem cells-derived cerebral organoids

Radiation induces DNA and protein damage and free radical formation, effectively establishing cellular senescence in a variety of models. We demonstrate the effects of two known pleiotropic drugs following gamma radiation damage in neurosphere/cerebral organoid system based on human embryonic stem c...

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Published in:Molecular biology reports 2019-02, Vol.46 (1), p.1343-1348
Main Authors: Shakhbazau, Antos, Danilkovich, Natalya, Seviaryn, Ihar, Ermilova, Tatyana, Kosmacheva, Svetlana
Format: Article
Language:English
Subjects:
Acidification
Animal Anatomy
Animal Biochemistry
Antibiotics
Biomedical and Life Sciences
Deoxyribonucleic acid
DNA
DNA damage
Embryo cells
Embryos
Histology
Life Sciences
Metabolic activation
Metabolic rate
Metabolism
Minocycline
Morphology
Organoids
Rapamycin
Senescence
Short Communication
Stem cells
Ventricle
Ventricles (cerebral)
γ Radiation
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cited_by cdi_FETCH-LOGICAL-c372t-92e3db7486a44cd3121214bdbf965984249d4dfc881783addf05d0ec7be42ab43
cites cdi_FETCH-LOGICAL-c372t-92e3db7486a44cd3121214bdbf965984249d4dfc881783addf05d0ec7be42ab43
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container_title Molecular biology reports
container_volume 46
creator Shakhbazau, Antos
Danilkovich, Natalya
Seviaryn, Ihar
Ermilova, Tatyana
Kosmacheva, Svetlana
description Radiation induces DNA and protein damage and free radical formation, effectively establishing cellular senescence in a variety of models. We demonstrate the effects of two known pleiotropic drugs following gamma radiation damage in neurosphere/cerebral organoid system based on human embryonic stem cells. mTORC1 repression by rapamycin prior to irradiation, or metabolic activation by minocycline after irradiation, partially rescues neuroepithelium integrity, neurite-growing capacity, ventricle formation and extracellular acidification rate as an integral measure of metabolic output. Cerebral organoid model thus provides valid and robust readouts for radiation studies in a complex 3D setting.
doi_str_mv 10.1007/s11033-018-4552-6
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subjects Acidification
Animal Anatomy
Animal Biochemistry
Antibiotics
Biomedical and Life Sciences
Deoxyribonucleic acid
DNA
DNA damage
Embryo cells
Embryos
Histology
Life Sciences
Metabolic activation
Metabolic rate
Metabolism
Minocycline
Morphology
Organoids
Rapamycin
Senescence
Short Communication
Stem cells
Ventricle
Ventricles (cerebral)
γ Radiation
title Effects of minocycline and rapamycin in gamma-irradiated human embryonic stem cells-derived cerebral organoids
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