Assessment of mycotoxin contamination in maize and wheat stored in silos using two sampling processes

Mycotoxin contamination of stored cereals often occurs in a highly heterogeneous manner, necessitating the use of representative sampling to minimise analytical errors. The objective of this study was to compare mycotoxin analysis in stored maize and wheat using two sampling processes. Samples were...

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Bibliographic Details
Published in:World mycotoxin journal 2018-01, Vol.11 (4), p.531-538
Main Authors: Mallmann, A.O, Oliveira, M.S, Dilkin, P, Vidal, J.K, Meinerz, G.R, Almeida, C.A.A, Mallmann, C.A
Format: Article
Language:English
Subjects:
Aflatoxins
Cereals
Coefficient of variation
Contamination
Corn
Deoxynivalenol
Fumonisins
Grain silos
Mathematical analysis
Pneumatic probes
RESEARCH ARTICLE
Sampling
Variability
Wheat
Zearalenone
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Summary:Mycotoxin contamination of stored cereals often occurs in a highly heterogeneous manner, necessitating the use of representative sampling to minimise analytical errors. The objective of this study was to compare mycotoxin analysis in stored maize and wheat using two sampling processes. Samples were obtained from four maize silos and two wheat silos. A pneumatic probe was introduced in the centre and at the four central points of each quadrant, from the top to the bottom of the silo (12 m). For sampling process A, this was divided into three samples (upper third, middle third and lower third of the silo height). No sample subdivision took place for sampling process B. LC-MS/MS was used for analysis of aflatoxins (AF), fumonisins (FB), zearalenone (ZEA) and deoxynivalenol (DON) in maize and DON and ZEA in wheat. Sampling procedures were compared with respect to the variability of the collected data. AF, FB, ZEA and DON were detected in 77.5, 100.0, 56.7 and 0.0% of the maize samples, respectively, and the mean concentration differed significantly between silos. In wheat, 100.0 and 97.5% of the samples were contaminated with DON and ZEA, respectively, and there was no significantly difference in mean concentration between silos. There was no significant difference (P>0.05) in the coefficients of variation (CVs) of AF (54.9 and 58.6%), FB (19.4 and 27.3%) and ZEA (68.9 and 85.5%) between sampling processes A and B in maize silos. The DON CV in sampling process A (10.1%) was lower (P
ISSN:1875-0710
1875-0796
DOI:10.3920/WMJ2018.2314