Methodological Features of the Morphometric Characterization of the Synaptoarchitectonics of the Human Neocortex by Immunofluorescent Detection of Neuromodulin

Objective. To study the possibility of using GAP-43 for morphometric characterization of synaptoarchitectonics. Materials and methods. Studies used immunofluorescent detection of neuromodulin (GAP-43) by confocal microscopy and automated computer image analysis of layer V (field 4) of the human brai...

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Published in:Neuroscience and behavioral physiology 2019, Vol.49 (1), p.103-108
Main Authors: Stepanov, A. S., Akulinin, V. A., Stepanov, S. S., Avdeev, D. B.
Format: Article
Language:English
Subjects:
Antibodies
Automation
Behavioral Sciences
Biomedical and Life Sciences
Biomedicine
Computer terminals
Confocal microscopy
Digital imaging
GAP-43 protein
Hypotheses
Image detection
Image processing
Microscopy
Neocortex
Neurobiology
Neurosciences
Particle size
Presynapse
Synapses
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container_end_page 108
container_issue 1
container_start_page 103
container_title Neuroscience and behavioral physiology
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creator Stepanov, A. S.
Akulinin, V. A.
Stepanov, S. S.
Avdeev, D. B.
description Objective. To study the possibility of using GAP-43 for morphometric characterization of synaptoarchitectonics. Materials and methods. Studies used immunofluorescent detection of neuromodulin (GAP-43) by confocal microscopy and automated computer image analysis of layer V (field 4) of the human brain ( n = 4) using ImageJ 1.46. Results. Immunofluorescent detection of GAP-43 identified the distribution of axodendritic and axosomatic synapses, the total area of axon terminals, and the number densities of intermediate and large terminals. Conclusions. A necessary condition for obtaining accurate data is having digital images with sufficient resolution (600–900 pixels per inch). These results need to be considered in studies of the synaptoarchitectonics of the neocortex using immunohistochemical methods for investigating synapse structure.
doi_str_mv 10.1007/s11055-018-0701-6
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subjects Antibodies
Automation
Behavioral Sciences
Biomedical and Life Sciences
Biomedicine
Computer terminals
Confocal microscopy
Digital imaging
GAP-43 protein
Hypotheses
Image detection
Image processing
Microscopy
Neocortex
Neurobiology
Neurosciences
Particle size
Presynapse
Synapses
title Methodological Features of the Morphometric Characterization of the Synaptoarchitectonics of the Human Neocortex by Immunofluorescent Detection of Neuromodulin
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