In vivo magnetic resonance imaging of injected mesenchymal stem cells in rat myocardial infarction; simultaneous cell tracking and left ventricular function measurement

To determine whether magnetic resonance imaging (MRI) can enable magnetically labeled mesenchymal stem cell (MSC) tracking and simultaneous in vivo functional data acquisition in rat models of myocardial infarction. Superparamagnetic iron oxide-laden human MSCs were injected into rat myocardium infa...

Full description

Saved in:
Bibliographic Details
Published in:The International Journal of Cardiovascular Imaging 2009-04, Vol.25 (Suppl 1), p.99-109
Main Authors: Kim, Young Jin, Huh, Yong-Min, Choe, Kyu Ok, Choi, Byoung Wook, Choi, Eun Jeong, Jang, Yangsoo, Lee, Jae Myun, Suh, Jin-Suck
Format: Article
Language:English
Subjects:
Animals
Cardiac Imaging
Cardiology
Cell Movement
Cells, Cultured
Contrast Media
Dextrans
Disease Models, Animal
Electrocardiography
Ferrosoferric Oxide
Humans
Imaging
Iron
Magnetic Resonance Imaging, Cine
Magnetite Nanoparticles
Male
Medicine
Medicine & Public Health
Mesenchymal Stem Cell Transplantation
Myocardial Infarction - pathology
Myocardial Infarction - physiopathology
Myocardial Infarction - surgery
Myocardium - pathology
Original Paper
Oxides
Radiology
Rats
Rats, Sprague-Dawley
Stroke Volume
Time Factors
Ventricular Function, Left
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To determine whether magnetic resonance imaging (MRI) can enable magnetically labeled mesenchymal stem cell (MSC) tracking and simultaneous in vivo functional data acquisition in rat models of myocardial infarction. Superparamagnetic iron oxide-laden human MSCs were injected into rat myocardium infarcted by cryoinjury 3 weeks after myocardial infarction. The control group received cell-free media injection. Before injection and for 3 months after, in vivo serial MRI was performed. Electrocardiography-gated gradient echo sequence MRI and cine MRI were performed for in vivo cell tracking and assessing cardiac function using left ventricular ejection fraction (LVEF), respectively. MRI revealed a persistent signal-void representing iron-laden MSCs until ten post-injection weeks. Serial follow-up MRI revealed that LVEF was significantly higher in the MSC injection group than in the control group. We conclude that MRI enables in vivo tracking of injected cells and evaluation of the long-term therapeutic potential of MSCs for myocardial infarction.
ISSN:1569-5794
1573-0743
1875-8312
DOI:10.1007/s10554-008-9407-0