Docosahexaenoic Acid Enhances Segregation of Lipids between Raft and Nonraft Domains: ^sup 2^H-NMR Study

Solid-state ^sup 2^H-NMR of [^sup 2^H^sub 31^]-N-palmitoylsphingomyelin ([^sup 2^H^sub 31^]16:0SM, PSM*), supplemented by differential scanning calorimetry, was used for the first time, to our knowledge, to investigate the molecular organization of the sphingolipid in 1:1:1 mol mixtures with 1-palmi...

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Bibliographic Details
Published in:Biophysical journal 2008-07, Vol.95 (1), p.203
Main Authors: Soni, Smita P, LoCascio, Daniel S, Liu, Yidong, Williams, Justin A, Bittman, Robert, Stillwell, William, Wassall, Stephen R
Format: Article
Language:English
Subjects:
Fatty acids
Lipids
Molecular biology
NMR
Nuclear magnetic resonance
Online Access:Get full text
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Summary:Solid-state ^sup 2^H-NMR of [^sup 2^H^sub 31^]-N-palmitoylsphingomyelin ([^sup 2^H^sub 31^]16:0SM, PSM*), supplemented by differential scanning calorimetry, was used for the first time, to our knowledge, to investigate the molecular organization of the sphingolipid in 1:1:1 mol mixtures with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (16:0-18:1PE, POPE) or 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (16:0-22:6PE, PDPE) and cholesterol. When compared with ^sup 2^H-NMR data for analogous mixtures of [^sup 2^H^sub 31^]16:0-18:1PE (POPE*) or[^sup 2^H^sub 31^]16:0-22:6PE (PDPE*) with egg SM and cholesterol, molecular interactions of oleic acid (OA) versus docosahexaenoic acid (DHA) are distinguished, and details of membrane architecture emerge. SM-rich, characterized by higher-order, and PE-rich, characterized by lower-order, domains
ISSN:0006-3495
1542-0086