Molecular design and synthesis of new dithiocarbazate complexes; crystal structure, bioactivities and nano studies

• Published in: RSC advances 2018-12, Vol.8 (73), p.41795-4189
• Main Authors: Yekke-ghasemi, Zahra, Takjoo, Reza, Ramezani, Mohammad, Mague, Joel T
• Format: Article
• Language: English
• Subjects: Binding sites; Biocompatibility; Chemical synthesis; Chemistry; Coordination compounds; Crystal structure; Crystallography; Crystals; Diffraction patterns; Fluorescence; Free energy; Infrared radiation; Mass spectrometry; NMR; Nuclear magnetic resonance; Quenching; Serum albumin; Single crystals; Spectrophotometry; Toxicity; Ultrasonic testing; X ray powder diffraction; X-ray diffraction
• ISSN: 2046-2069; 2046-2069
• DOI: 10.1039/c8ra07100d

The syntheses of a new set of metal complexes MoO 2 L′(CH 3 OH), VOL′(CH 3 O)(CH 3 OH), , , SnL′Cl 2 and SnL′I 2 with a new ligand (L = (2,2′(disulfanediylbis((ethylthio)methylene)bis(hydrazin-2-yl-1-ylidene)bis(methanylylidene)) diphenol; L′ = S -ethyl-3-(2-hydroxyphenyl)methylenedithiocarbazate are described along with characterization by elemental analysis, mass spectrometry, spectroscopic (IR, 1 H- and 13 C-NMR) and TGA techniques. The crystal structures of compounds were determined by single crystal X-ray diffraction analysis and compared to powder X-ray diffraction (PXRD) patterns of the nano complexes obtained using ultrasonic methods. The PXRD results indicate that the compounds synthesized by ultrasonic methods have high crystallinity. The compounds were evaluated in an in vitro cytotoxicity study with two human cancer cell lines. The results of this study revealed that all complexes exhibit good cytotoxic activity when compared to the clinical drug, cisplatin. Interaction of the samples with human serum albumin (HSA) was investigated using fluorescence spectrophotometric methods and the Stern-Volmer quenching constant ( K SV ) and free energy changes (Δ G ) were calculated at 298 K. The fluorescence quenching method is used to determine the number of binding sites ( n ) and association constants ( K a ) at the same temperatures. New metal complexes are synthesized and characterized by elemental analysis, mass spectrometry, spectroscopic, TGA and X-ray techniques. The compounds were evaluated in an in vitro cytotoxicity. Interaction of the samples with HSA was investigated.