Fluctuations in ribosomal RNA gene content and nucleolar activity in the cambial region of Abies balsamea (Pinaceae) shoots during reactivation

Tissue was collected from the vascular cambial region of 1-year-old balsam fir shoots over an 11-week period during which cambial reactivation occurred. The amount of rDNA (ribosomal RNA genes) relative to total genomic DNA was determined by quantitative slot blots for three trees, one of which show...

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Bibliographic Details
Published in:American journal of botany 1994-11, Vol.81 (11), p.1384-1389
Main Authors: Lloyd, A.D. (University of New Brunswick, Fredericton, New Brunswick, Canada), Mellerowicz, E.J, Chow, C.H, Riding, R.T, Little, C.H.A
Format: Article
Language:English
Subjects:
ABIES BALSAMEA
ADN
ANATOMIA DE LA PLANTA
ANATOMIE VEGETALE
ARN RIBOSOMAL
ARN RIBOSOMIAL
Botany
CAMBIUM
Cell nucleolus
Cell nucleus
Cellular biology
DIVISION CELLULAIRE
DIVISION CELULAR
DNA
DNA probes
DORMANCE
DORMICION
Genes
GENETICA
GENETIQUE
Genomics
NUCLEOLE
NUCLEOLOS
Phloem
Physiology, Biochemistry, and Cell Biology
Ribonucleic acid
Ribosomal DNA
RNA
rRNA genes
TALLO
TIGE
Trees
VARIACION ESTACIONAL
VARIATION SAISONNIERE
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Summary:Tissue was collected from the vascular cambial region of 1-year-old balsam fir shoots over an 11-week period during which cambial reactivation occurred. The amount of rDNA (ribosomal RNA genes) relative to total genomic DNA was determined by quantitative slot blots for three trees, one of which showed a 3-week delay in reactivation. In addition, nucleolar activity was estimated by measuring nucleolar volume, number, and staining intensity. Relative rRNA gene content increased transiently prior to the onset of cambial cell periclinal division. Nucleolar volume also increased transiently, but 1-2 weeks prior to the maximal relative rDNA value. The increases in relative rDNA and nucleolar activity were delayed in the tree in which reactivation was late. We interpret these changes as reflecting the amplification and loss of genes encoding rRNA to facilitate cambial cell reactivation
ISSN:0002-9122
1537-2197
DOI:10.1002/j.1537-2197.1994.tb15622.x