Development of a peroxide biodetector for a direct detection of biofilms produced by catalase-positive bacteria on food-contact surfaces

Detection of biofilms on food-contact surfaces is essential for establishing sanitization procedures and avoiding microbial contamination of food products. The objective of our study was to develop a peroxide biodetector, to detect and reveal the presence of biofilms. A positive reaction was observe...

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Bibliographic Details
Published in:CYTA: journal of food 2018-01, Vol.16 (1), p.506-515
Main Authors: Ripolles-Avila, Carolina, Ríos-Castillo, Abel G., Rodríguez-Jerez, José J.
Format: Article
Language:English
Subjects:
Bacteria
biodetector
Biofilms
Biopelículas
Catalase
detección
detection
E coli
Food
Food contamination
food hygiene
Food industry
Food processing industry
Food production
Foodborne pathogens
higiene alimentaria
Listeria
Listeria monocytogenes
Microbial contamination
Microorganisms
método rápido
Peroxide
Polypropylene
Pseudomonas aeruginosa
rapid method
Salmonella
Stainless steel
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Summary:Detection of biofilms on food-contact surfaces is essential for establishing sanitization procedures and avoiding microbial contamination of food products. The objective of our study was to develop a peroxide biodetector, to detect and reveal the presence of biofilms. A positive reaction was observed when biodetector bubbles were formed on stainless steel and polypropylene surfaces. A range of microorganisms able to form biofilms was evaluated. Catalase-positive bacteria: Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica ser. Typhimurium and Cronobacter sakazakii were employed as well as the catalase-negative bacterium: Lactobacillus brevis. With the exception of C. sakazakii, all catalase-positive foodborne pathogens forming the biofilms showed a positive detection by the biodetector, being 10 4 CFU cm −2 the minimum microbial load detected. The strongest positive reaction was for P. aeruginosa. Results demonstrated the potential of this biodetector to detect biofilms, particularly when used as a tool in the food industry.
ISSN:1947-6337
1947-6345
DOI:10.1080/19476337.2017.1418434