An ^sup 86^Y-Labeled Mirror-Image Oligonucleotide: Influence of Y-DOTA Isomers on the Biodistribution in Rats

A mirror-image oligonucleotide (L-RNA) was radiolabeled with the positron emitting radionuclide ...Y (t... = 14.7 h) via the bifunctional chelator approach. DOTA-modification of the L-RNA (sequence: 5'-aminohexyl UGA CUG ACU GAC-3'; MW 3975) was performed using (S)-p-SCN-Bn-DOTA. ...Y radi...

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Bibliographic Details
Published in:Bioconjugate chemistry 2008-04, Vol.19 (4), p.928
Main Authors: Schlesinger, Joern, Koezle, Inge, Bergmann, Ralf, Tamburini, Sergio, Bolzati, Cristina, Tisato, Francesco, Noll, Bernhard, Klussman, Sven, Vonhoff, Stefan, Wuest, Frank, Pietzsch, Hans-Juergen, Steinbach, Joerg
Format: Article
Language:English
Subjects:
Chemical compounds
Chemical synthesis
Radiometry
Ribonucleic acid
RNA
Rodents
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Summary:A mirror-image oligonucleotide (L-RNA) was radiolabeled with the positron emitting radionuclide ...Y (t... = 14.7 h) via the bifunctional chelator approach. DOTA-modification of the L-RNA (sequence: 5'-aminohexyl UGA CUG ACU GAC-3'; MW 3975) was performed using (S)-p-SCN-Bn-DOTA. ...Y radiolabeling of the DOTA-L-RNA produced more than one species as evidenced by HPLC radiometric detection. For the identification of the ...Y-labeled L-RNA, the structural analogue nonradioactive precursor [Y((S)-p-NH...-Bn-DOTA)]... was synthesized. Two coordination isomers were separated via HPLC adopting the square antiprismatic (SAP) and the twisted square antiprismatic (TSAP) geometry, respectively. Their stereochemical configuration in the solution state was assessed by NMR and circular dichroism spectroscopy. Both [Y((S)-p-NH...-Bn-DOTA)]... isomers were converted into isothiocyanate derivatives [Y((S)-p-SCN-Bn-DOTA)]... and conjugated to the L-RNA. The identity of the [...Y-DOTA]-L-RNA species was finally established by comparison of the radiometric (...Y) and UV-visible chromatographic profiles. Biodistribution studies in Wistar rats showed minor changes in the biodistribution profile of the [...Y((S)-p-NH...-Bn-DOTA)]... complex isomers, while no significant differences were observed for the [...Y-DOTA]-L-RNA isomers. High renal excretions were found for the [...Y((S)-p-NH...-Bn-DOTA)]... complex isomers as well as for the L-RNA isomers. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:1043-1802
1520-4812