Polygalacturonase secreted by yeasts from Brazilian semi-arid environments

Microbial pectinolytic enzymes are known to play a commercially important role in a number of industrial processes. The objective of this study was to investigate the extracellular polygalacturonases of yeasts isolated from Brazilian semi-arid environments. Among the 250 colonies tested, only 33 pro...

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Bibliographic Details
Published in:International journal of food sciences and nutrition 2009-01, Vol.60 (s7), p.72-80
Main Authors: Oliveira, Rodrigo Q., Rosa, Carlos A., Uetanabaro, Ana Paula T., Azeredo, Antônio, Neto, Aristóteles Góes, Assis, Sandra A.
Format: Article
Language:English
Subjects:
biotechnology
Brazil
Cells
D1/D2 domain
Databases, Nucleic Acid
Desert Climate
Enzymes
Food science
Food Technology - methods
Food-Processing Industry - methods
Fungal Proteins - genetics
Fungal Proteins - metabolism
Fungal Proteins - secretion
Fungi - classification
Fungi - enzymology
Fungi - genetics
Fungi - isolation & purification
Genes, Fungal
pectinolytic enzymes
Pectins - metabolism
Phylogeny
Polygalacturonase - genetics
Polygalacturonase - metabolism
Polygalacturonase - secretion
Polymerase Chain Reaction
RNA, Ribosomal - chemistry
RNA, Ribosomal - genetics
Sequence Homology, Nucleic Acid
Studies
Symbiosis
Yeast
Yeasts - enzymology
Yeasts - genetics
Yeasts - isolation & purification
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Summary:Microbial pectinolytic enzymes are known to play a commercially important role in a number of industrial processes. The objective of this study was to investigate the extracellular polygalacturonases of yeasts isolated from Brazilian semi-arid environments. Among the 250 colonies tested, only 33 produced extracellular polygalacturonases: Aureobasidium pullulans (18 isolates), Candida boidinii (one isolate), Trichosporonoides sp. (three isolates), Kluyveromyces marxianus (one isolate), Cryptococcus liquefaciens (one isolate), Pseudozyma sp. (four isolates), and yeast-like related to fungal endophyte (five isolates). The highest activity of polygalacturonase was observed in Pseudozyma sp. CCMB 300 (14.17±0.08 µmol acid galacturonic released/min/mg protein). This study shows the potential of yeasts and yeast-like organisms isolated from Brazilian semi-arid environments to produce pectinolytic enzymes.
ISSN:0963-7486
1465-3478
DOI:10.1080/09637480802534517