Polymorphism in TMEM95, SUGT1 and RASGRP2 genes in Russian Simmental cattle population

Monitoring of recessive genetic disorders in cattle populations has a great importance due to the serious economic damages they cause. The most studied breed is Holsteins, where more than 20 such disorders were identified. Less is known about the distribution of genetic defects in other cattle breed...

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Bibliographic Details
Published in:Journal of animal science 2018-12, Vol.96, p.125-125
Main Authors: Kostyunina, O, Filipchenko, A, nara, M, Sermyagin, A, Zinovieva, N
Format: Article
Language:English
Subjects:
Abnormalities
Animal populations
Bovidae
Cattle
Deoxyribonucleic acid
Disorders
DNA
Embryos
Farms
Fertility
Fetuses
Gene polymorphism
Genes
Genetic disorders
Genotype & phenotype
Genotyping
Haplotypes
Hemophilia
Mutation
Polymerase chain reaction
Polymorphism
Population genetics
Populations
Restriction fragment length polymorphism
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Summary:Monitoring of recessive genetic disorders in cattle populations has a great importance due to the serious economic damages they cause. The most studied breed is Holsteins, where more than 20 such disorders were identified. Less is known about the distribution of genetic defects in other cattle breeds. Simmental is the second largest population after Holsteins in Russia. At least nine different LoF mutations were found in this breed worldwide. In our work, we evaluated the frequency of LoF mutations in TMEM95, SUGT1 and RASGRP2 genes associated with heritable abnormalities BMS (Bovine Male Subfertility), FH4 (Fleckvieh Haplotype 4) and TP (thrombopathia) in Simmental cattle population in Russia. BMS is revealed in complete or partial absence of bulls' fertility, FH4 leads to decrease in the conception rate and early embryonic losses, and TP causes the hemophilia of animals. The genotyping was performed using PCR-RFLP and pyrosequencing approaches. In total, 139 sires and 177 cows from different farms of the central region of Russia were genotyped for casual mutations in TMEM95, SUGT1 and RASGRP2 genes. The ratio of carriers of LoF-mutations among sires and cows was 3.60% and 6.21% for TMEM95, 5.04% and 2.26% for SUGT1 and 4.32% and 2.26% for RASGRP2, respectively. Our data shows the presence of BMS, FH4 and TP carriers both in sire and in cow populations of Russian Simmental cattle. DNA diagnostics can be an effective way to reduce the frequency of carriers and to exclude occurrence of affected fetuses due to the mating of carries of the same disorders. The research was supported by the Federal Agency for Scientific Organizations within theme No. AAAA-A18-118021590138-1.
ISSN:0021-8812
1525-3163