Silver nanoparticles as dietary additive influences the microbial digestive ecosystem in weaned piglets

Following previous research studies of the effect of silver nanoparticles (AgN) on digestive microbiota in pigs, we aimed to study the microbial changes induced by the addition of AgN on the digestive tract site (ileum or caecum) in weaning piglets and its influence on fermentation. Sixteen piglets...

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Bibliographic Details
Published in:Journal of animal science 2018-12, Vol.96, p.326-326
Main Authors: de la Fuente, G, Suárez-Belloch, J, Latorre, M, Fondevila, M
Format: Article
Language:English
Subjects:
Acetic acid
Additives
Data processing
Diet
Fermentation
Gastrointestinal tract
Hogs
Ileum
Microbiota
Microorganisms
Nanoparticles
Population (statistical)
Propionic acid
rRNA 16S
Silver
Taxa
Weaning
Weight
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Summary:Following previous research studies of the effect of silver nanoparticles (AgN) on digestive microbiota in pigs, we aimed to study the microbial changes induced by the addition of AgN on the digestive tract site (ileum or caecum) in weaning piglets and its influence on fermentation. Sixteen piglets weaned at 28 days (7.08 kg average weight) were given a prestarter diet, without (CT, n=6) or with 20 ppm metallic AgN (SIL, n=10) for 14 days. Then, piglets were slaughtered and their ileal and caecal contents sampled for their 16s rRNA sequencing using llumina MiSeq. Taxonomic assignment was performed using BLASTN against a RDP database data and analysed using R and JMP statistical packages. Ileal (6.60 vs. 6.65) and caecal (5.57 vs. 5.58) pH did not differ between CT and SIL. Volatile fatty acid concentration at the ileum was low, but in the caecum molar proportion of acetate was higher (0.577 vs. 0.461), and propionate lower (0.236 vs. 0.318), in CT than SIL. Addition of AgN decreased the ileal richness (P=0.002) but increased Lactobacillus sp. (P=0.07) in the ileum and reductions in Clostridium sp. (P=0.09 in ileum and P=0.03 in caecum) were observed when AgN were added. Taxa most influenced by addition of AgN included Treponema porcinum and Acitenobacter sp. at ileum and Desulfovibrio sp. and Ruminococcus albus at caecum. Spearman correlations between fermentation parameters and relevant taxa in both ileum and caecum also differed when AgN was added. Silver nanoparticles exert a differential effect on the microbial population depending on the digestive site (research supported by the project POCTEFA EFA-183/16).
ISSN:0021-8812
1525-3163