Quantitative determination of taxine B in body fluids by LC-MS-MS

A new specific and sensitive LC-MS-MS method for the detection of taxine B and isotaxine B, the main toxic pseudo-alkaloids from yew (Taxus sp.), in biological samples (blood, urine, gastric content) was developed. Biological samples were prepared for LC-MS-MS by means of solid-phase extraction (SPE...

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Bibliographic Details
Published in:International journal of legal medicine 2006-11, Vol.120 (6), p.346-351
Main Authors: Frommherz, L, Kintz, P, Kijewski, H, Köhler, H, Lehr, M, Brinkmann, B, Beike, J
Format: Article
Language:English
Subjects:
Adult
Alkaloids - analysis
Animals
Chromatography
Chromatography, High Pressure Liquid
Forensic Toxicology
Gastrointestinal Contents - chemistry
Horses
Humans
Isomerism
Legal medicine
Mass Spectrometry
Methods
Molecular Structure
Plant Extracts - analysis
Poisoning
Poisoning - diagnosis
Reference materials
Sulfuric acid
Taxoids - analysis
Toxicity
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Summary:A new specific and sensitive LC-MS-MS method for the detection of taxine B and isotaxine B, the main toxic pseudo-alkaloids from yew (Taxus sp.), in biological samples (blood, urine, gastric content) was developed. Biological samples were prepared for LC-MS-MS by means of solid-phase extraction (SPE) procedure and yielded a recovery of 86%. Chromatographic separation was achieved using an RP(18) column. Detection of taxine B and isotaxine B was performed using multiple reaction monitoring with m/z 584.2 as precursor ion, i.e. [M+H](+), of both isomers and m/z 194.3 and m/z 107.1 as product ions after collision-induced dissociation. Docetaxel was applied as internal standard. The method was fully validated for the analysis of blood samples. Linearity was proven in the range from 0.1-500 ng/g. The limit of detection and the limit of quantitation are 0.4 and 2 ng/g, respectively. The method was applied to the determination of taxine B and isotaxine B in four fatal cases (two humans, two horses) with suspected yew intoxication. Blood levels were 105, 168, 174 and 212 ng/g.
ISSN:0937-9827
1437-1596
DOI:10.1007/s00414-005-0071-9