pH-Dependent fluorescence from firefly oxyluciferin in agarose thin films

The yellow-green light emitted by fireflies is one of the most prominent examples of bioluminescence. Firefly oxyluciferin, the emitting molecule, is labile in alkaline solutions, and its structure is strongly affected by solvent polarity and pH. Previous studies have suggested that variations in th...

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Bibliographic Details
Published in:New journal of chemistry 2019, Vol.43 (3), p.1122-1126
Main Authors: Lui, Nathan M., Schramm, Stefan, Naumov, Panče
Format: Article
Language:English
Subjects:
Anions
Bioluminescence
Environmental effects
Fireflies
Fluorescence
Polarity
Thin films
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Summary:The yellow-green light emitted by fireflies is one of the most prominent examples of bioluminescence. Firefly oxyluciferin, the emitting molecule, is labile in alkaline solutions, and its structure is strongly affected by solvent polarity and pH. Previous studies have suggested that variations in the active site conditions are likely contributors to the color of bioluminescent emission. Herein, we incorporate firefly oxyluciferin into an agarose matrix to emulate the enzyme active site. Self-supporting, lightweight thin films were fabricated by solution casting and spectroscopically characterized. The previously described acidochromism of oxyluciferin is conserved in the thin films. The bathochromic shift observed in alkaline conditions results from the formation of the oxyluciferin dianion. This study demonstrates an alternative approach to investigating environmental effects on bioluminescent molecules.
ISSN:1144-0546
1369-9261
DOI:10.1039/C8NJ05469J