Microfluidic device with a push–pull sequential solution-exchange function for affinity sensing

A simple valveless microfluidic device consisting of a reaction chamber and eight flow channels was fabricated for the detection of proteins. The efficient exchange of solutions in the reaction chamber was achieved by sequentially introducing solutions into the reaction chamber and removing them fro...

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Bibliographic Details
Published in:Microfluidics and nanofluidics 2019-02, Vol.23 (2), p.1-8, Article 19
Main Authors: Pramanik, Shishir Kanti, Suzuki, Hiroaki
Format: Article
Language:English
Subjects:
Analytical Chemistry
Automation
Biomedical Engineering and Bioengineering
Channels
Dependence
Detection
Dust
Engineering
Engineering Fluid Dynamics
Exchanging
Flow channels
Fluorescence
Fluorescence microscopy
Hydrophobicity
Immunoassay
Immunoassays
Interleukin 2
Interleukins
Manufacturing
Microfluidic devices
Nanotechnology and Microengineering
Polydimethylsiloxane
Proteins
Research Paper
Silicone resins
Solutions
Viscosity
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Summary:A simple valveless microfluidic device consisting of a reaction chamber and eight flow channels was fabricated for the detection of proteins. The efficient exchange of solutions in the reaction chamber was achieved by sequentially introducing solutions into the reaction chamber and removing them from the reaction chamber through the same flow channels. The influence of the hydrophobicity of the reaction chamber and the viscosity of a blocking solution on solution exchange was investigated. The analytical performance of the device was examined by detecting human interleukin 2 (IL-2) using a sandwich fluorescence immunoassay, with the amount of immuno-complex formed in the reaction chamber monitored by fluorescence microscopy. The approach facilitated the rapid detection of IL-2, in which the total assay time was less than 30 min. A clear dependence of fluorescence intensity on IL-2 concentration was observed over the 125 pg/mL–2.0 ng/mL range, with a detection limit of 105 pg/mL. To promote automation, a simple mechanism that exchanges solutions by the push–pull movement of a PDMS diaphragm was also demonstrated.
ISSN:1613-4982
1613-4990
DOI:10.1007/s10404-019-2188-z