Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators

We prepared water soluble, biocompatible fluorescent turn-on pH nanosensors and characterized their behavior as a function of changes in pH. The response relies on a halochromic reaction of a spirorhodamineamide derived from the bright and highly chemically and photo-stable rhodamine 6G, encapsulate...

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Bibliographic Details
Published in:Photochemical & photobiological sciences 2019-01, Vol.18 (1), p.155-165
Main Authors: Di Paolo, M, Roberti, M. J, Bordoni, A. V, Aramendía, P. F, Wolosiuk, A, Bossi, M. L
Format: Article
Language:English
Subjects:
Aqueous environments
Biochemistry
Biocompatibility
Biomaterials
Cellular structure
Chemistry
Confocal microscopy
Core-shell structure
Encapsulation
Fluorescence
Halochromism
Kinetics
Localization
Lysosomes
Microscopy
Nanoparticles
Organic chemistry
pH effects
Physical Chemistry
Plant Sciences
Reaction kinetics
Rhodamine 6G
Silica
Silicon dioxide
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Summary:We prepared water soluble, biocompatible fluorescent turn-on pH nanosensors and characterized their behavior as a function of changes in pH. The response relies on a halochromic reaction of a spirorhodamineamide derived from the bright and highly chemically and photo-stable rhodamine 6G, encapsulated in core/nanoporous shell silica nanoparticles. The fluorescent sensors displayed a fast response in the pH range of intracellular compartments. The encapsulation conferred solubility in aqueous environments and biocompatibility. We assessed the two main properties of the sensor, namely the useful pH range and the kinetics of the response, and compared them to those of the free probe. We found that such properties are strongly dependent on the functionalization and position in the silica matrix relative to the core/shell structure. Finally, we demonstrated the cellular uptake of the nanosensors, and their localization in lysosomes of living cells, by fluorescence confocal microscopy. pH sensing by silica nanoparticles carrying a fluorescent turn-on spirorhodamineamide. Left/Right: Confocal imaging pH 7.4/2.0. Inset: SEM.
ISSN:1474-905X
1474-9092
DOI:10.1039/c8pp00133b