Protein changes in gastric epithelial cells RGM‐1 in response to Helicobacter pylori infection

Helicobacter pylori‐induced inflammation significantly increases the risk of gastric cancer. To investigate the role of H. pylori infection in gastric epithelial cell carcinogenesis, flow cytometry was used to analyze the apoptosis of gastric epithelial cells infected by H. pylori. Next, LTQ MS mass...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2019-03, Vol.120 (3), p.3197-3202
Main Authors: Lv, Zengfa, Zhao, Lizhi, Jin, Weimin
Format: Article
Language:English
Subjects:
Apoptosis
Bioinformatics
Carcinogenesis
Carcinogens
Epithelial cells
ErbB protein
Flow cytometry
Gastric cancer
gastric epithelial cells
Helicobacter pylori
Infections
inflammatory response
Localization
Mass spectrometry
Mass spectroscopy
p53 Protein
Proteins
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Summary:Helicobacter pylori‐induced inflammation significantly increases the risk of gastric cancer. To investigate the role of H. pylori infection in gastric epithelial cell carcinogenesis, flow cytometry was used to analyze the apoptosis of gastric epithelial cells infected by H. pylori. Next, LTQ MS mass spectrometry (MS) was applied to identify protein changes in gastric epithelial cells infected with H. pylori, and then bioinformatics was adopted to analyze the cellular localization and biological function of differential proteins. LTQ MS/MS successfully identified identified 22 differential proteins successfully, including 20 host‐cell proteins and two H. pylori bacterial proteins. Also, human proteins were located in all areas of cells and involved in various cell biological functions. The oncogene proteins p53, p16, and C‐erbB‐2 proteins in H. pylori‐infected RGM‐1 cells were remarkably increased from the analysis by Western blot analysis. H. pylori infection of gastric epithelial cells leads to changes in various protein components in the cell, and enhances the expression of oncogene proteins, thereby increasing the possibility of possibility of carcinogenesis of H. pylori infection.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.27585