Exercise training ameliorates bleomycin‐induced epithelial mesenchymal transition and lung fibrosis through restoration of H2S synthesis

• Published in: Acta Physiologica 2019-02, Vol.225 (2), p.n/a
• Main Authors: Du, Shu‐Fang, Wang, Xiu‐Li, Ye, Chang‐Lin, He, Ze‐Jia, Li, Dong‐Xia, Du, Bai‐Ren, Liu, Yu‐Jian, Zhu, Xiao‐Yan
• Format: Article
• Language: English
• Subjects: Actin; Bleomycin; Catenin; Clinical trials; Collagen (type I); E-cadherin; epithelial‐mesenchymal transition; exercise training; Fibrosis; Fitness training programs; Glycogen; Glycogen synthase kinase 3; H2S; Hydrogen sulfide; Hydroxyproline; Immunofluorescence; Kinases; Localization; Lung diseases; lung fibrosis; Mesenchyme; Phosphorylation; Receptor density; Rodents; Signal transduction; Smad protein; Smad2 protein; Smad3 protein; Smad4 protein; Smooth muscle; TGF‐β1; Trachea; Transforming growth factor; Transforming growth factor-b1
• ISSN: 1748-1708; 1748-1716
• DOI: 10.1111/apha.13177

Aims Clinical trials have shown the beneficial effects of exercise training against pulmonary fibrosis. This study aimed to investigate whether prophylactic intervention with exercise training attenuates lung fibrosis via modulating endogenous hydrogen sulphde (H2S) generation. Methods First, ICR mice were allocated to Control, Bleomycin, Exercise, and Bleomycin + Exercise groups. Treadmill exercise began on day 1 and continued for 4 weeks. A single intratracheal dose of bleomycin (3 mg/kg) was administered on day 15. Second, ICR mice were allocated to Control, Bleomycin, H2S, and Bleomycin + H2S groups. H2S donor NaHS (28 μmol/kg) was intraperitoneally injected once daily for 2 weeks. Results Bleomycin‐treated mice exhibited increased levels of collagen deposition, hydroxyproline, collagen I, transforming growth factor (TGF)‐β1, Smad2/Smad3/low‐density lipoprotein receptor‐related proteins (LRP‐6)/glycogen synthase kinase‐3β (GSK‐3β) phosphorylation, and Smad4/β‐catenin expression in lung tissues (P