Molecular characterization of the Cassava brown streak virus coat protein

A partial sequence of 1114 nucleotides of a virus from cassava brown streak diseased (CBSD) material was obtained. Alignment of the predicted amino acid sequence with those of other members of the Potyviridae showed closest identity with the coat protein of Sweet potato mild mottle virus (genus Ipom...

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Bibliographic Details
Published in:Plant pathology 2001-08, Vol.50 (4), p.527-534
Main Authors: Monger, W. A., Seal, S., Isaac, A. M., Foster, G. D.
Format: Article
Language:English
Subjects:
Biological and medical sciences
cassava
Cassava brown streak virus
Fundamental and applied biological sciences. Psychology
Ipomovirus
Microbiology
Morphology, structure, chemical composition, physicochemical properties
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
Potyviridae
Systematics
Systematics. Structure, properties and multiplication. Genetics
Virology
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Summary:A partial sequence of 1114 nucleotides of a virus from cassava brown streak diseased (CBSD) material was obtained. Alignment of the predicted amino acid sequence with those of other members of the Potyviridae showed closest identity with the coat protein of Sweet potato mild mottle virus (genus Ipomovirus). The predicted amino acid sequence has one open reading frame with a 3′ untranslated region of 144 nucleotides and a poly(A) tail. The expressed protein was shown to cross‐react with an antiserum raised previously to a virus isolated from CBSD material. Evidence presented suggests that CBSD is caused by Cassava brown streak virus, a tentative member of the genus Ipomovirus, as this virus is consistently found associated with CBSD.
ISSN:0032-0862
1365-3059
DOI:10.1046/j.1365-3059.2001.00589.x