High-Resolution Nano-Liquid Chromatography with Tandem Mass Spectrometric Detection for the Bottom-Up Analysis of Complex Proteomic Samples

Liquid chromatography coupled with mass spectrometric detection is one of the major technologies used for protein sequencing, identification, and quantification. This review provides an introduction of the current state-of-the-art technology in peptide profiling using nano-liquid chromatography–mass...

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Bibliographic Details
Published in:Chromatographia 2019-01, Vol.82 (1), p.101-110
Main Authors: Dams, Magali, Dores-Sousa, José Luís, Lamers, Robert-Jan, Treumann, Achim, Eeltink, Sebastiaan
Format: Article
Language:English
Subjects:
50th Anniversary Commemorative Issue
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Chromatography
Ionization
Ions
Laboratory Medicine
Liquid chromatography
Mass spectrometry
Peptides
Pharmacy
Proteins
Proteomics
Review
Scientific imaging
Spectroscopy
Workflow
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Summary:Liquid chromatography coupled with mass spectrometric detection is one of the major technologies used for protein sequencing, identification, and quantification. This review provides an introduction of the current state-of-the-art technology in peptide profiling using nano-liquid chromatography–mass spectrometry applied to large-scale protein or proteome analysis. In particular, different aspects of the bottom-up proteomics workflow are covered, including aspects of sample preparation such as protein digestion, nanoflow gradient reversed-phase chromatographic separation, LC–MS interfacing via electrospray ionization, tandem mass spectrometry of digests, protein identification via database searches, and finally peptide quantitation.
ISSN:0009-5893
1612-1112
DOI:10.1007/s10337-018-3647-5