Biological characteristics and lytic effectiveness of phages isolated from domestic wastewater against indigenous Salmonella spp

• Published in: Journal of environmental biology 2019-01, Vol.40 (1), p.53-60
• Main Authors: Budiarti, S., Sunarti, R.N., Listya, S.A.P., Rusmana, I.
• Format: Article
• Language: English
• Subjects: Antibiotics; Antimicrobial agents; Bacteria; Biological effects; Buffers; Cattle; Dairy farms; Diarrhea; Disease; Domestic wastewater; Drug resistance; E coli; Electrophoresis; Environmental science; Food contamination & poisoning; Gel electrophoresis; Icosahedral phase; Magnesium; Morphology; Nanotechnology; Pathogens; Patients; pH effects; Phages; Physical characteristics; Population; Proteins; Salmonella; Sodium; Sodium dodecyl sulfate; Sodium lauryl sulfate; Stability analysis; Wastewater
• ISSN: 0254-8704; 2394-0379
• DOI: 10.22438/jeb/40/1/MRN-804

Aim : This study was carried out to isolate and study the effectiveness of lytic phage from domestic wastewater to reduce the population of Salmonella spp. in patients suffering from diarrhea and to characterize biological phages. Methodology : The lytic phages from several domestic wastewater were identified using a transmission electron microscope to know morphological phages. After identifying the molecularweight protein by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, to know the effectiveness, the lytic phages were infected to Salmonella spp. from diarrheal disease patients and non-pathogenic Escherichia coll. Phage stability on thermal, pH, and buffer was then analyzed to determine the biological characteristics. Results : Three lytic phages (F-SB1, F-SB2, and F-SB3), successfully isolated from domestic wastewater, showed an icosahedral head with a short or long tail as their morphological characteristic. These phages were morphologically similar to the phages of family Siphoviridae, Myoviridae and Podoviridae. The three isolated lytic phages were stable at 27 °C to 37 °C, pH 4-7 in sodium magnesium buffer and effectively decreased the population of Salmonella spp., however could not lyse E. coli. Interpretation : All the isolated lytic phages in this study can contribute as cocktail phages in decreasing the population Salmonella spp.