Identification and physical mapping of genes expressed in the corpus luteum in cattle1

Summary A representational difference analysis was performed to identify genes expressed in the corpus luteum of cattle. The corpus luteum is an ovarian structure that is essential for the establishment and maintenance of pregnancy. Knowledge of gene expression and function of corpus luteum will be...

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Bibliographic Details
Published in:Animal genetics 2003-10, Vol.34 (5), p.325-333
Main Authors: Bønsdorff, T., Eggen, A., Gautier, M., Åsheim, H.‐C., Rønningen, K., Lingaas, F., Olsaker, I.
Format: Article
Language:English
Subjects:
cattle
corpus luteum
expressed sequence tags
ovary
radiation hybrid panel
representational difference analysis
somatic cell hybrid panel
subtractive hybridization
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Summary:Summary A representational difference analysis was performed to identify genes expressed in the corpus luteum of cattle. The corpus luteum is an ovarian structure that is essential for the establishment and maintenance of pregnancy. Knowledge of gene expression and function of corpus luteum will be important to improve fertility in humans and domestic animals. Housekeeping genes were removed from the corpus luteum representation (tester) using skeletal muscle as the subtracting agent (driver). A total of 80 clones of the final subtraction product were analysed by sequencing and 11 new bovine gene sequences were identified (pBTCL1–11). The sequences were mapped to segments of 10 different chromosomes using a somatic cell hybrid panel and a radiation hybrid panel. With one exception the locations are in agreement with published comparative maps of cattle and man. Expression in corpus luteum was verified by RT–PCR for all the 11 clones.
ISSN:0268-9146
1365-2052
DOI:10.1046/j.1365-2052.2003.01023.x