Thymosin (beta4) sequesters the majority of G-actin in resting human polymorphonuclear leukocytes

To determine whether chemoattractant induced actin polymerization results from an inactivation of thymosin beta4, the G-actin sequestering activity of supernatants prepared from resting and chemoattractant stimulated cells was measured by comparing the rates of pyrenyl-actin polymerization from fila...

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Bibliographic Details
Published in:The Journal of cell biology 1992-12, Vol.119 (5), p.1261
Main Authors: Cassimeris, Lynne, Safer, Daniel, Nachmias, Vivianne T, Zigmond, S H
Format: Article
Language:English
Subjects:
Cellular biology
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Summary:To determine whether chemoattractant induced actin polymerization results from an inactivation of thymosin beta4, the G-actin sequestering activity of supernatants prepared from resting and chemoattractant stimulated cells was measured by comparing the rates of pyrenyl-actin polymerization from filament pointed ends. Pyrenyl actin polymerization was inhibited to a greater extent in supernatants from stimulated cells and these results are consistent with thymosin beta4 being released as G-actin polymerizes.
ISSN:0021-9525
1540-8140