Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance

Aims The aim of this study was to investigate whether the overexpression of NifA and NodD regulators contribute to the symbiotic improvement of chickpea mesorhizobia. Methods The native strains V-15b, ST-2, and PMI-6 were transformed with extra copies of nifA or nodD genes and several plants trial w...

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Published in:Plant and soil 2019-03, Vol.436 (1/2), p.607-621
Main Authors: da-Silva, José Rodrigo, Menéndez, Esther, Eliziário, Fernando, Mateos, Pedro F., Alexandre, Ana, Oliveira, Solange
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Chick peas
Chickpeas
Ecology
Gene expression
Genes
Genetic aspects
Genetic research
Hydroponics
Life Sciences
Nodules
Observations
Plant genetics
Plant growth
Plant Physiology
Plant Sciences
REGULAR ARTICLE
Regulators
Soil Science & Conservation
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cited_by cdi_FETCH-LOGICAL-c380t-691e6d4d916da79f4ecfb2c35de2cb3049fb9f69744cb1001fd907881e68a7403
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container_issue 1/2
container_start_page 607
container_title Plant and soil
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creator da-Silva, José Rodrigo
Menéndez, Esther
Eliziário, Fernando
Mateos, Pedro F.
Alexandre, Ana
Oliveira, Solange
description Aims The aim of this study was to investigate whether the overexpression of NifA and NodD regulators contribute to the symbiotic improvement of chickpea mesorhizobia. Methods The native strains V-15b, ST-2, and PMI-6 were transformed with extra copies of nifA or nodD genes and several plants trial were performed. Results Plant growth assays showed that nifA overexpression was able to improve the symbiotic effectiveness of V-15b, while nodD overexpression lead to the improvement of ST-2 and PMI-6. Hydroponic assays showed that plants inoculated with V15b nifA + and PMI6 nodD + started developing nodules earlier than those inoculated with the corresponding control strains. In addition, the number of nodules was always higher in plants inoculated with the strains overexpressing the symbiotic genes. Analysis of histological sections of nodules formed by V15b nifA + showed a more developed fixation zone when compared with control. On the other hand, nodules induced by PMI6 nodD + did not show a senescent zone, which was observed in nodules from plants inoculated with the control strain. Plants inoculated with PMI6 nodD + and ST2 nodD + showed a higher number of infection threads than the corresponding control inoculations. Conclusion These results indicate that overexpressing nifA and nodD may be an important tool to achieve the improvement of the symbiotic performance of mesorhizobia.
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Methods The native strains V-15b, ST-2, and PMI-6 were transformed with extra copies of nifA or nodD genes and several plants trial were performed. Results Plant growth assays showed that nifA overexpression was able to improve the symbiotic effectiveness of V-15b, while nodD overexpression lead to the improvement of ST-2 and PMI-6. Hydroponic assays showed that plants inoculated with V15b nifA + and PMI6 nodD + started developing nodules earlier than those inoculated with the corresponding control strains. In addition, the number of nodules was always higher in plants inoculated with the strains overexpressing the symbiotic genes. Analysis of histological sections of nodules formed by V15b nifA + showed a more developed fixation zone when compared with control. On the other hand, nodules induced by PMI6 nodD + did not show a senescent zone, which was observed in nodules from plants inoculated with the control strain. Plants inoculated with PMI6 nodD + and ST2 nodD + showed a higher number of infection threads than the corresponding control inoculations. Conclusion These results indicate that overexpressing nifA and nodD may be an important tool to achieve the improvement of the symbiotic performance of mesorhizobia.</description><identifier>ISSN: 0032-079X</identifier><identifier>EISSN: 1573-5036</identifier><identifier>DOI: 10.1007/s11104-019-03950-0</identifier><language>eng</language><publisher>Cham: Springer Science + Business Media</publisher><subject>Biomedical and Life Sciences ; Chick peas ; Chickpeas ; Ecology ; Gene expression ; Genes ; Genetic aspects ; Genetic research ; Hydroponics ; Life Sciences ; Nodules ; Observations ; Plant genetics ; Plant growth ; Plant Physiology ; Plant Sciences ; REGULAR ARTICLE ; Regulators ; Soil Science &amp; Conservation</subject><ispartof>Plant and soil, 2019-03, Vol.436 (1/2), p.607-621</ispartof><rights>Springer Nature Switzerland AG 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Plant and Soil is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-691e6d4d916da79f4ecfb2c35de2cb3049fb9f69744cb1001fd907881e68a7403</citedby><cites>FETCH-LOGICAL-c380t-691e6d4d916da79f4ecfb2c35de2cb3049fb9f69744cb1001fd907881e68a7403</cites><orcidid>0000-0001-7033-4186</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/48703624$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/48703624$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,58238,58471</link.rule.ids></links><search><creatorcontrib>da-Silva, José Rodrigo</creatorcontrib><creatorcontrib>Menéndez, Esther</creatorcontrib><creatorcontrib>Eliziário, Fernando</creatorcontrib><creatorcontrib>Mateos, Pedro F.</creatorcontrib><creatorcontrib>Alexandre, Ana</creatorcontrib><creatorcontrib>Oliveira, Solange</creatorcontrib><title>Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance</title><title>Plant and soil</title><addtitle>Plant Soil</addtitle><description>Aims The aim of this study was to investigate whether the overexpression of NifA and NodD regulators contribute to the symbiotic improvement of chickpea mesorhizobia. 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Plants inoculated with PMI6 nodD + and ST2 nodD + showed a higher number of infection threads than the corresponding control inoculations. Conclusion These results indicate that overexpressing nifA and nodD may be an important tool to achieve the improvement of the symbiotic performance of mesorhizobia.</abstract><cop>Cham</cop><pub>Springer Science + Business Media</pub><doi>10.1007/s11104-019-03950-0</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-7033-4186</orcidid></addata></record>
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subjects Biomedical and Life Sciences
Chick peas
Chickpeas
Ecology
Gene expression
Genes
Genetic aspects
Genetic research
Hydroponics
Life Sciences
Nodules
Observations
Plant genetics
Plant growth
Plant Physiology
Plant Sciences
REGULAR ARTICLE
Regulators
Soil Science & Conservation
title Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance
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