Trypsin-modified-fused-silica capillary microreactor for peptide mapping by capillary zone electrophoresis

An analytical procedure involving trypsin immobilized on the inner surface of a 50 microm-i.d. fused-silica capillary has been developed for on-line digestion of minute amounts of protein. Laser-induced fluorescence was used to detect the separated NDA-labeled tryptic peptides in the digest.

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1992-07, Vol.64 (14), p.1610-1613
Main Authors: Amankwa, Lawrence N, Kuhr, Werner G
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemistry
Fluorescence
Fundamental and applied biological sciences. Psychology
Lasers
Proteins
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Description
Summary:An analytical procedure involving trypsin immobilized on the inner surface of a 50 microm-i.d. fused-silica capillary has been developed for on-line digestion of minute amounts of protein. Laser-induced fluorescence was used to detect the separated NDA-labeled tryptic peptides in the digest.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00038a019