Electrophoresis Separation in Open Microchannels. A Method for Coupling Electrophoresis with MALDI-MS

The separation of biological mixtures in open microchannels using electrophoresis with rapid and simple coupling to mass spectrometry is introduced. Rapid open-access channel electrophoresis employs microchannels that are manufactured on microchips. Separation is performed in the open channels, and...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2001-05, Vol.73 (9), p.2147-2151
Main Authors: Liu, Jun, Tseng, Ken, Garcia, Ben, Lebrilla, Carlito B, Mukerjee, Eric, Collins, Scott, Smith, Rosemary
Format: Article
Language:English
Subjects:
Aminoacids, peptides. Hormones. Neuropeptides
Analytical chemistry
Analytical, structural and metabolic biochemistry
Animals
beta-Cyclodextrins
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Cyclodextrins - analysis
Cyclodextrins - isolation & purification
Electrophoresis, Capillary - instrumentation
Electrophoresis, Capillary - methods
Exact sciences and technology
Fourier Analysis
Fundamental and applied biological sciences. Psychology
Gentisates
Hydroxybenzoates - chemistry
Kallidin - analysis
Kallidin - isolation & purification
Male
Oligosaccharides - analysis
Oligosaccharides - isolation & purification
Other chromatographic methods
Proteins
Rats
Rats, Sprague-Dawley
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Spectrum analysis
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Summary:The separation of biological mixtures in open microchannels using electrophoresis with rapid and simple coupling to mass spectrometry is introduced. Rapid open-access channel electrophoresis employs microchannels that are manufactured on microchips. Separation is performed in the open channels, and the chips are transferred to a matrix-assisted laser desorption/ionization (MALDI) source after the solvent is evaporated. The matrix (2,5-dihydroxybenzoic acid) is placed in the solution with the run buffer before the separation of the analyte components. After separation, the solvent is evaporated and the microchip is ready for MALDI-MS analysis. The microchip is placed directly into a specially designed ion source of an external source Fourier transform mass spectrometry instrument. Separation of simple mixtures containing oligosaccharides and peptides is shown.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac001326t