Positive-Ion Analysis of Boropeptides by Chemical Ionization and Liquid Secondary Ionization Mass Spectrometry

Techniques for the characterization of two boronic acid peptides as their cyclic boronate ester derivatives by positive-ion ammonia chemical ionization (CI) and positive-ion liquid secondary ionization (LSIMS) are described and results presented. These techniques avoid the complications introduced b...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1999-04, Vol.71 (8), p.1574-1578
Main Authors: Haas, Michael J, Blom, Karl F, Schwarz, Carl H
Format: Article
Language:English
Subjects:
Acids
Analytical chemistry
Chemicals
Chemistry
Exact sciences and technology
Ions
Spectrometric and optical methods
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Summary:Techniques for the characterization of two boronic acid peptides as their cyclic boronate ester derivatives by positive-ion ammonia chemical ionization (CI) and positive-ion liquid secondary ionization (LSIMS) are described and results presented. These techniques avoid the complications introduced by the thermally induced processes that boronic acids may undergo when the mass spectrometric characterizations of free boronic acids are attempted. Derivatizations for CI analysis were accomplished via a simple benchtop method using several polyfunctional nucleophilic derivatizing agents (ethylene glycol, glycerol, et al.), while derivatizations for LSIMS analysis were accomplished via both benchtop and previously established in situ methods using the same derivatizing agents. Certain previously held misconceptions about the LSIMS mass spectrometry of boronic acids are examined.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac9810112